文献类型：期刊文献

中文题名：两种蛀干昆虫消化酶组成和活性比较

英文题名：Comparison of Components and Activity of Digestive Enzymes between Two Xylophagous Insects

作者：王健敏[1] 陈晓鸣[1] 冯颖[1] 段兆尧[2]

第一作者：王健敏

机构：[1]中国林业科学研究院资源昆虫研究所国家林业局资源昆虫培育与利用重点实验室;[2]云南省林业科学院

年份：2007

卷号：20

期号：2

起止页码：170-175

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：云南省自然科学基金重点项目"利用蛀干害虫作为指示昆虫诊断森林健康研究"

语种：中文

中文关键词：蛀干昆虫;松墨天牛;纵坑切梢小蠹;消化酶

外文关键词：xylophagous insect; Monochamus alternatus ; Tomicus piniperda ; digestive enzyme

分类号：S763

摘要：研究了松墨天牛幼虫、纵坑切梢小蠹幼虫和成虫消化酶的主要种类和活性。分析结果表明,松墨天牛幼虫有10种消化酶,纵坑切梢小蠹幼虫和成虫有8种消化酶。松墨天牛幼虫的消化酶活性大小排列顺序为:果胶酶>淀粉酶>Cx酶>半纤维素酶>转化酶>β-1,4-葡萄糖苷酶>C1酶>脂肪酶>类胰蛋白酶>胃蛋白酶;小蠹幼虫和成虫不含Cx酶和-β1,4-葡萄糖苷酶,活性顺序均为:果胶酶>淀粉酶>半纤维素酶>转化酶>C1酶>类胰蛋白酶>脂肪酶>胃蛋白酶。在55℃、pH值8.6时,果胶酶活性最高。
The components and activity of digestive enzymes extracted from larvae of Monochamus alternatus, larvae and adults of Tomicus piniperda were analyzed in this paper. The results revealed that, there were 10 digestive enzymes in Monochamus alternatus and 8 in Tomicus piniperda. The activity order of enzymes of larvae of Monochamus alternatus arranged as pectinase 〉 amylase 〉 endo-β-1,4-glucanase （Cx-ase） 〉 hemicellulase 〉 invertase 〉 β-1,4- glucosidase 〉 exo-β-1,4-glucanase （Cl-ase） 〉 lipase 〉 trypsinlike enzyme 〉 pepsin; and for larvae and adults of Tomicus piniperda, the activity order of enzymed arranged as pectinase 〉 amylase 〉 hemicellulase 〉 invertase 〉 exo-β- 1,4-glucanase（Cl-ase） 〉 trypsinlike enzyme 〉 lipase 〉 pepsin. There were no Cx-ase and β-1,4-glucosidase for both larvae and adults of Tomicus piniperda. The result pectinase had the highest activity under 55 ℃ and pH = 8.6.