文献类型：期刊文献

英文题名：The aqueous assembly preparation of OPs-AgNPs with phenols from olive mill wastewater and its mechanism on antimicrobial function study

作者：Qi, Zhiwen[1,3] Xue, Xingying[1,3] Zhou, Hao[1] Yuan, Hua[1] Li, Wenjun[1] Yang, Guliang[2] Xie, Pujun[1] Wang, Chengzhang[1]

第一作者：Qi, Zhiwen;齐志文

通信作者：Xie, PJ[1];Yang, GL[2]

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Natl Engn Lab Biomass Chem Utilizat, Nanjing 210042, Jiangsu, Peoples R China;[2]Cent South Univ Forestry & Technol, Food Sci & Engn Coll, Natl Engn Lab Rice & Prod Proc, Changsha 410004, Hunan, Peoples R China;[3]Coinnovat Ctr Efficient Proc & Utilizat Forest Re, Nanjing 210042, Jiangsu, Peoples R China

年份：2022

卷号：376

外文期刊名：FOOD CHEMISTRY

收录：;EI(收录号：20215211399028);Scopus(收录号：2-s2.0-85121829517);WOS:【SCI-EXPANDED(收录号:WOS:000741200300006)】；

基金：We are grateful for financial support from National Natural Science Foundation of China (Grant No.: 31800614) .

语种：英文

外文关键词：Olive mill wastewater phenols; AgNPs; Antimicrobial; Escherichia coli ATCC 23815; Membrane disruption; Ca2+-ATPase

摘要：To valorise olive mill wastewater phenols (OPs) potentially applied in food preservation, a novel stable and regularly spherical OPs-AgNPs (D-avg = 78 nm) were successfully assembled in aqueous solution under the optimized conditions (pH 8.0, 5 mM AgNO3, 35C and 30 min). The results of antimicrobial zone diameters indicated that 50 mu g/mL of promising OPs-AgNPs presented excellent antimicrobial effects. Especially, the cell wall damages of E. coli ATCC 23,815 were caused when OPs-AgNPs concentration was exceeded its MIC (8.58 mu g/mL). Also, a significant down-regulating of the Ca2+-ATPase activity in E. coli was revealed, and the intracellular Ca2+ concentrations were thus decreased from 12.5 to 1.35 mu g/mL after a treatment for 3 h. The apoptosis level of E. coli was significantly increased more than the control (55.13% of OPs-AgNPs vs 9.90% of control). In sum, OPs exerts enhanced antimicrobial function via penetrating cell membrane and targeting Ca2+-ATPase after chelated with AgNPs.