文献类型：期刊文献

中文题名：羟基酪醇纳米脂质体冻干粉的制备及其性能评价

英文题名：Preparation and Performance Evaluation of Hydroxytyrosol Nanoliposome Freeze-dried Powder

作者：李文君[1,2,3] 王成章[1] 雷建都[2] 周昊[1]

第一作者：李文君

机构：[1]中国林业科学研究院林产化学工业研究所,江苏省生物质能源与材料重点实验室,国家林业和草原局林产化学工程重点实验室,林木生物质低碳高效利用国家工程研究中心,江苏南京210042;[2]北京林业大学材料科学与技术学院,北京100083;[3]南京林业大学江苏省林业资源高效加工利用协同创新中心,江苏南京210037

年份：2023

卷号：43

期号：5

起止页码：125-132

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：江苏省生物质能源与材料重点实验室开放基金项目(JSBEM-S-201810)。

语种：中文

中文关键词：冷冻干燥法;再分散性;正交试验;冻干保护剂;稳定贮藏

外文关键词：freeze-drying method;redispersibility;orthogonal test;freeze-drying protective agent;stable storage

分类号：TQ35

摘要：为了使羟基酪醇(HT)纳米脂质体在低温下能够长期贮存,在制备HT纳米脂质体悬浮液的基础上,通过冷冻干燥法制备HT纳米脂质体冻干粉,以其外观、再分散性、平均粒径和包封率为主要指标,筛选冻干工艺条件,并采用单因素联合正交试验筛选冻干保护剂,评价其稳定性。研究结果表明:-50℃下速冻8 h,冷冻干燥72 h,冻干保护剂为质量比4∶2∶1的D-海藻糖/蔗糖/甘露醇溶液且溶液质量分数为10%时,所得的冻干粉外观松散、光滑、平整饱满(外观+++),振荡1 min内能复溶成均匀悬浮液且无不溶颗粒或团块(再分散性+++),含水率3.28%±0.23%,平均粒径(125.24±1.58)nm,包封率69.74%±0.94%。透射电镜观察发现:冻干粉复溶后纳米粒子外观圆整、表面光滑、大小均一。贮藏稳定性考察发现:低温更利于冻干粉的稳定贮藏,4℃下贮藏6个月时,冻干粉稳定性较好,性能没有明显变化。
In order to make hydroxytyrosol(HT)nano-liposomes stored at low temperature for a long time,the freeze-dried powder of HT nano-liposomes was prepared by freeze-drying method based on the preparation of the suspension of HT nano-liposomes.The freeze-drying process conditions were selected based on its appearance,redispersability,particle size and encapsulation rate as the main indexes,and the freeze-drying protective agent was screened by single factor test combined with orthogonal test to evaluate its stability.The results showed that the obtained freeze-dried powder was loose,smooth,flat and full appearance(appearance+++)when the freeze-dried protective agent was m(D-trehalose)∶m(sucrose)∶m(mannitol)=4∶2∶1 and the solution mass fraction was 10%at-50℃for 8 h and freeze-drying for 72 h.The freeze-dried powder could redissolve into uniform suspension within 1 min of oscillation without soluble particles or clumps(redispersability+++),with water content of 3.28%±0.23%,the average particle size of(125.24±1.58)nm,and the encapsulation rate of 69.74%±0.94%.Transmission electron microscopy showed that the particles were round in appearance,smooth in surface and uniform in size.The stability study found that low temperature was more conducive to the stable storage of freeze-dried powder.When it was stored at 4℃for 6 months,the freeze-dried powder exhibited better stability and no significant change in performance.