文献类型：期刊文献

中文题名：油茶籽油中角鲨烯含量的气相色谱法测定

英文题名：Determination of squalene in camellia seed oil by gas chromatography

作者：钟冬莲[1] 汤富彬[1] 沈丹玉[1] 莫润宏[1] 丁明[1]

第一作者：钟冬莲

机构：[1]中国林业科学研究院亚热带林业研究所,国家林业局经济林产品质量检验检测中心,富阳311400

年份：2011

卷号：30

期号：11

起止页码：104-106

中文期刊名：分析试验室

外文期刊名：Chinese Journal of Analysis Laboratory

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：油茶籽油;角鲨烯;皂化;气相色谱法

外文关键词：Camellia seed oil ; Squalene ; Saponification ; Gas chromatography

分类号：O657.7

摘要：建立了油茶籽油中角鲨烯含量测定的气相色谱检测方法。油茶籽油样品经氢氧化钾-乙醇溶液皂化,石油醚萃取净化,经HP-5色谱柱分离,气相色谱仪(GC-FID)检测,外标法定量。在5～200g/L线性范围内线性良好,在1.0～3.0g/kg添标水平,平均回收率在93.7%～101.3%,相对标准偏差在1.7%～6.8%,方法的最低检出限为0.0013g/kg。方法适用于食用植物油中角鲨烯含量的测定。
An analytical method was established for the determination of squalene in Camellia seed oil by gas chromatography. The Camellia seed oil samples were saponified with potassium hydroxide and extracted with petroleum ether, andthen the extraction solution was separated on a HP -5 column and determined with gas chromatography （GC-FID）. The linear range of the method was 5 - 200g/L （r = 0. 9999） The recoveries of squalene ranged from 93. 7% to 101.3% at fortification levels of 1.0 -3. 0 g/kg, and the relative standard deviation varied from 1.7% to 6. 8%. Limit of detection was 0. 0013 g/kg. The proposed method is suitable for the determination of squalene in other edible vegetable oils.