文献类型：期刊文献

英文题名：Genome sequencing and analysis of the paclitaxel-producing endophytic fungus Penicillium aurantiogriseum NRRL 62431

作者：Yang, Yanfang[1] Zhao, Hainan[2] Barrero, Roberto A.[3] Zhang, Baohong[4] Sun, Guiling[5,6] Wilson, Iain W.[7] Xie, Fuliang[4] Walker, Kevin D.[8,9] Parks, Joshua W.[10] Bruce, Robert[10] Guo, Guangwu[11] Chen, Li[11] Zhang, Yong[12] Huang, Xin[13] Tang, Qi[14,15] Liu, Hongwei[1] Bellgard, Matthew I.[3] Qiu, Deyou[1] Lai, Jinsheng[2] Hoffman, Angela[10]

第一作者：杨艳芳

通信作者：Qiu, DY[1]|[a000598d4c276c5f9aea6]邱德有;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China;[2]China Agr Univ, Coll Agr Biotechnol, Beijing 100094, Peoples R China;[3]Murdoch Univ, Ctr Comparat Genom, Perth, WA 6150, Australia;[4]E Carolina Univ, Dept Biol, Greenville, NC 27858 USA;[5]Southwest Forestry Univ, Sch Life Sci, Kunming 650224, Peoples R China;[6]Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China;[7]CSIRO Plant Ind, Canberra, ACT 2001, Australia;[8]Michigan State Univ, Dept Chem, E Lansing, MI 48824 USA;[9]Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA;[10]Univ Portland, Dept Chem, Portland, OR 97203 USA;[11]BGI Shenzhen, Shenzhen 518000, Peoples R China;[12]China Agr Univ, Dept Plant Pathol, Beijing 100094, Peoples R China;[13]Chinese Acad Inspect & Quarantine, Inst Plant Quarantine, Beijing 100029, Peoples R China;[14]Hunan Agr Univ, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China;[15]Hunan Agr Univ, Natl Chinese Med Herbs Hunan Technol Ctr, Changsha 410128, Hunan, Peoples R China

年份：2014

卷号：15

期号：1

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000332573300001)】；

基金：This work was supported by a grant for National non-profit Research Institutions of Chinese Academy of Forestry and NSFC grant 31170628. We thank Prof. Gary A. Strobel for useful discussions.

语种：英文

外文关键词：Penicillium aurantiogriseum NRRL 62431; Paclitaxel; Taxol (TM); Endophytic fungi; Genome sequence; Horizontal gene transfer

摘要：Background: Paclitaxel (Taxol (TM)) is an important anticancer drug with a unique mode of action. The biosynthesis of paclitaxel had been considered restricted to the Taxus species until it was discovered in Taxomyces andreanae, an endophytic fungus of T. brevifolia. Subsequently, paclitaxel was found in hazel (Corylus avellana L.) and in several other endophytic fungi. The distribution of paclitaxel in plants and endophytic fungi and the reported sequence homology of key genes in paclitaxel biosynthesis between plant and fungi species raises the question about whether the origin of this pathway in these two physically associated groups could have been facilitated by horizontal gene transfer. Results: The ability of the endophytic fungus of hazel Penicillium aurantiogriseum NRRL 62431 to independently synthesize paclitaxel was established by liquid chromatography-mass spectrometry and proton nuclear magnetic resonance. The genome of Penicillium aurantiogriseum NRRL 62431 was sequenced and gene candidates that may be involved in paclitaxel biosynthesis were identified by comparison with the 13 known paclitaxel biosynthetic genes in Taxus. We found that paclitaxel biosynthetic gene candidates in P. aurantiogriseum NRRL 62431 have evolved independently and that horizontal gene transfer between this endophytic fungus and its plant host is unlikely. Conclusions: Our findings shed new light on how paclitaxel-producing endophytic fungi synthesize paclitaxel, and will facilitate metabolic engineering for the industrial production of paclitaxel from fungi.