文献类型：期刊文献

英文题名：Simple ultrasonic-assisted approach to prepare polysaccharide-based aerogel for cell research and histocompatibility study

作者：Hu, Xinyu[1,2,3,4,5] Wang, Yongmei[1] Zhang, Liangliang[1] Xu, Man[1]

第一作者：Hu, Xinyu;胡新宇

通信作者：Hu, XY[1]|[a0005881e11db1c0efa81]胡新宇;

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forestry Prod, Key Lab Biomass Energy & Mat, Nanjing 210042, Jiangsu, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry New Technol, Beijing 100091, Peoples R China;[3]Coinnovat Ctr Efficient Proc & Utilizat Forest Re, Nanjing 210042, Jiangsu, Peoples R China;[4]Natl Forestry & Grassland Adm, Key Lab Chem Engn Forest Prod, Beijing 100714, Peoples R China;[5]Natl Engn Lab Biomass Chem Utilizat, Nanjing 210042, Peoples R China

年份：2021

卷号：188

起止页码：411-420

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20242616366611);Scopus(收录号：2-s2.0-85112377434);WOS:【SCI-EXPANDED(收录号:WOS:000696989700003)】；

基金：This work was supported by the Fundamental Research Funds of CAF under the Grant CAFYBB2020QB007 and the Key Lab. of Biomass En-ergy and Material, Jiangsu Province (KLBEM) under the Grant JSBEM-S-201904.

语种：英文

外文关键词：Aerogel; Ultrasonic-assisted self-assembly; Salecan; Cationic starch; Cell adhesion and proliferation; Histocompatibility study

摘要：Salecan, a water-soluble microbial polysaccharide with attractive biocompatible characteristics, is very suitable for aerogel fabrication. However, the practical application of salecan-based aerogels for cell culture was limited by complicated preparation method, lack of cell anchorage signals, and the ability to modulate this properly. Here, a smart aerogel was designed by ultrasonic-assisted self-assembly of salecan and cationic starch (CAS) without any organic and toxic crosslinkers. The ultrasound waves generated a marked impact on self-assemble process by means of ultrasonic cavitation. Aerogel network was produced by strong electrostatic attractions between the polysaccharides. Especially, salecan/CAS ratio can be precisely modulated to tailor the hydrophilicity, mechanical stiffness, and morphologic property. The specific surface area of the aerogels gradually increased with the increase in salecan/CAS ratio. These aerogels were non-cytotoxic, and the incorporation of salecan into them promoted cell-matrix interactions by directionally supporting cell adhesion and proliferation. Most strikingly, in vivo experiment revealed that the histological features in the main organs of the mice were similar to those observed in the PBS-treated control group, and no sign of the histopathological abnormality or tissue destruction was observed, indicating the excellent histocompatibility of the aerogels. This study offered a new and powerful avenue to fabricate functional biomaterial.