文献类型：期刊文献

英文题名：Identification and characterization of MYC transcription factors in Taxus sp.

作者：Yang Yanfang[1] Zhang Kaikai[1,2] Yang Liying[2] Lv Xing[2] Wu Ying[2] Liu Hongwei[3] Lu Qiang[4] Chen Duanfen[2] Qiu Deyou[1]

第一作者：杨艳芳

通信作者：Qiu, DY[1];Chen, DF[2]|[a000598d4c276c5f9aea6]邱德有;

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat State Forestry A, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Agr Univ Hebei, Coll Hort, Baoding 071001, Peoples R China;[3]Hebei Acad Sci, Hebei Engn & Technol Ctr Microbiol Control Main C, Biol Inst, Shijiazhuang 050081, Hebei, Peoples R China;[4]Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Beijing Natl Lab Mol Sci,Synthet & Fu, Beijing 100871, Peoples R China

年份：2018

卷号：675

起止页码：1-8

外文期刊名：GENE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000445169900001)】；

基金：This work was supported by National Natural Science Foundation of China (31570675, 31300567), a grant from the National Key Research and Development Program of China (2017YFD060070605), and a grant for National non-profit Research Institutions of Chinese Academy of Forestry (CAFYBB2018SY009).

语种：英文

外文关键词：Transcription factor; MYC; G-box; bHLH; Taxol; Taxus sp.

摘要：Myelocytomatosis (MYC) transcription factors (TFs) are key regulators of the jasmonic acid (JA) signaling pathway. In cell cultures, methyl jasmonate (MeJA) can improve the production of taxol, which is a complex terpenoid compound with an intense antitumor activity. However, the functions of MYC genes in Taxus sp. (yew trees) remain poorly known. Based on Taxus sp. transcriptome changes induced by MeJA, a TcMYC gene was isolated in a previous study. Here, we further characterized the TcMYC TF encoded by that gene and four other yew MYC TFs previously obtained. Three yew MYC TFs had the typical basic helix-loop-helix (bHLH)-MYC _N region, but the other two MYC did not, although all five presented the bHLH domain. TcMYC was localized to the nuclei, and phylogenetic analysis indicated that the yew MYC TFs were closely related to Arabidopsis thaliana MYC1/2 and maize R protein. The yeast one-hybrid assay showed that TcMYC binds the G-box of the promoter of taxane 5 alpha-hydroxylase. Transcript levels of TcMYC revealed that TcMYC was highly expressed in xylem and leaves, and up-regulated by drought and high-salinity stresses. Coronatine (COR) has recently been used as a new elicitor to improve the production of taxol in cell cultures; TcMYC was strongly expressed at 2 and 4 h after COR treatment, but decreased at 12 and 24 h. Overall, the results obtained here provide new insights into the potential regulatory roles of MYC TFs on taxol biosynthesis in yew trees.