文献类型：期刊文献

中文题名：美洲大蠊乙酸乙酯提取物的分离和抑菌活性分析

英文题名：Isolation of Ethyl Acetate Extract from Periplaneta americana and Its Antimicrobial Activity

作者：王奎[1] 冯颖[1] 孙龙[1] 何钊[1] 陈智勇[1]

机构：[1]中国林业科学研究院资源昆虫研究所国家林业局资源昆虫培育与利用重点实验室

年份：2013

卷号：26

期号：2

起止页码：163-166

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家林业局"西南特色昆虫资源及经济树种种质资源收集保存与良种繁育基地建设"项目

语种：中文

中文关键词：美洲大蠊;乙酸乙酯提取物;抑菌活性;不饱和脂肪酸

外文关键词：Periplaneta americana; ethyl acetate extract; antimicrobial activity; unsaturated fatty acid

分类号：S718.43

摘要：采用活性追踪法和柱层析法,以金黄色葡萄球菌、大肠杆菌等5种细菌为供试菌种,对美洲大蠊乙酸乙酯提取物进行分离和抑菌试验。研究发现:美洲大蠊乙酸乙酯提取物对供试的革兰氏阳性菌具有一定的抑制作用,其中,对枯草芽孢杆菌的抑菌活性最好;经硅胶柱层析初分离后,从乙酸乙酯提取物获得了9个具有抑菌活性的组分,其中,组分Fr7对5种供试菌均显示了较好的抑菌活性;组分Fr7及其相邻组分Fr8经硅胶和凝胶柱层析进一步分离纯化后,得到了7个具有抑菌活性的不饱和脂肪酸类化合物,均对藤黄微球菌表现出最好的抑菌活性。结果表明:美洲大蠊乙酸乙酯提取物的抑菌活性物质为不饱和脂肪酸类化合物,对供试的革兰氏阳性菌的抑菌活性较强。
Antimicrobial chemical components, which isolated from the ethyl acetate extract of Periplaneta americana L., were tested for the ability to inhibit Staphyloccocus aureus, Escherichia coli, etc. , based on bioassay-guided method. The results indicated that the ethyl acetate extract of P. americana showed some inhibiting effects to all gram-positive bacteria tested, and the best inhibition effect was against Bacillus subtilis. Nine factions were obtained after silica gel column chromatography. Among them, the faction Fr7 showed inhibiting effects to all bacteria tested. Applying the isolation method of silica gel column and gel chromatography, seven unsaturated fatty acids compounds were isolated from the faction Fr7 and Fr8. All these compounds showed the best inhibiting effects against Micrococ-cus luteus. The results suggested that unsaturated fatty acids were antimicrobial chemical components of the ethyl ac-etate extract from P. americana. The inhibition effects of the unsaturated fatty acids against gram-positive bacteria were stronger than against gram-negative bacteria.