文献类型：期刊文献

英文题名：Characterization and expression analysis of genes encoding Taxol biosynthetic enzymes in Taxus spp.

作者：Jiang, Luyuan[1,2] Zhang, Kaikai[1] Lu, Xing[3] Yang, Liying[2] Wang, Shuai[4] Chen, Duanfen[2] Yang, Yanfang[1] Qiu, Deyou[1]

第一作者：Jiang, Luyuan

通信作者：Yang, YF[1]

机构：[1]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat, State Key Lab Tree Genet & Breeding, State Forestry Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[2]Hebei Agr Univ, Coll Hort, Baoding 071001, Peoples R China;[3]Hebei Agr Univ, Coll Agron, Baoding 071001, Peoples R China;[4]Liaocheng Univ, Sch Pharm, Liaocheng 252000, Shandong, Peoples R China

年份：2021

卷号：32

期号：6

起止页码：2507-2515

外文期刊名：JOURNAL OF FORESTRY RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000607508000001)】；

基金：This work was supported by National Natural Science Foundation of China (31570675), a Grant from the National Key Research and Development Program of China (2017YFD060070605), and a Grant for National non-profit Research Institutions of Chinese Academy of Forestry (CAFYBB2018SY009).

语种：英文

外文关键词：Gene expression; Bioinformatics analysis; Acyltransferase; Cytochrome P450; Abiotic stress; Hormone

摘要：Taxol (Paclitaxel), an important anticancer drug, is derived at very low yields from Taxus (yew) species that grow very slowly. In the present study, thirteen genes that encode enzymes involved in Taxol biosynthesis in Taxus spp. were analyzed with bioinformatics methods, and their expression levels in different tissues and after cold and hormone treatments were also analyzed. The results indicated that many cis-elements related to abiotic stresses and hormones were found in the promoter sequences of the 8 genes involved in Taxol biosynthesis. Moreover, the 13 enzymes encoded by the target genes were located in different organelles and had many phosphorylation sites in the response proteins. The 13 genes were expressed highly either in roots or in stems, with lower transcripts in needles, and they were highly expressed after treatment with cold, gibberellin, methyl jasmonate or coronatine, consistent with predictions based on the bioinformatics analysis. These results suggest that the factors such as hormones and abiotic stresses stimulate taxane biosynthesis in yews, providing an important way to sustainably generate taxanes from yew trees or their cell cultures to improve Taxol yields.