文献类型：期刊文献

中文题名：紫外法和皮粉法测定塔拉单宁含量的对比研究

英文题名：Comparison on the Determination of Tara Tannin Content with UV Spectrophotometry and Hide Powder Method

作者：汤丽华[1] 张亮亮[1] 张禾[1]

第一作者：汤丽华

机构：[1]中国林业科学研究院林产化学工业研究所,江苏省生物质能源与材料重点实验室,国家林业和草原局林产化学工程重点实验室,林木生物质低碳高效利用国家工程研究中心,江苏省林业资源高效加工利用协同创新中心,江苏南京210042

年份：2022

卷号：56

期号：6

起止页码：31-35

中文期刊名：生物质化学工程

外文期刊名：Biomass Chemical Engineering

基金：江苏省生物质能源与材料重点实验室基本科研业务费(JSBEM-S-202005)。

语种：中文

中文关键词：紫外法;皮粉法;植物单宁;塔拉单宁

外文关键词：UV spectrophotometry;hide powder method;vegetable tannin;tara tannin

分类号：TQ35;TQ9

摘要：分别采用紫外法和皮粉法对5种不同含量的塔拉单宁进行对比研究,并利用SPSS软件对单宁含量测定结果进行显著性检验,结果表明:高纯度塔拉单宁(93%)分别用紫外法和皮粉法测定单宁含量时实验结果并未显著差异(P>0.05),而4种纯度为60%左右塔拉单宁的实验结果均具有显著性差异(P<0.05),皮粉法测得的单宁含量均比用紫外法测得的结果高,差值约为1.5%~2.0%,并由此计算出紫外法测定塔拉单宁含量计算公式中的矫正常数p为1.03。紫外-可见吸收光谱与HPLC分析结果表明:塔拉单宁与五倍子单宁的最大吸收峰均为276 nm,同质量浓度下塔拉单宁的吸收峰强度高于五倍子单宁,塔拉单宁成分出峰时间主要在20~40 min,五倍子单宁主要在30~45 min。
Five kinds of tara tannin with different contents were determined and compared by UV spectrophotometry and hide powder method,and the significance test of tannin content determination results was carried out by SPSS software.The results showed that there was no significant difference in the determination of tannin content of high-puritytaratannin(93%)measured by UV spectrophotometry and hide powder method(P>0.05).However,the experimental results of the four kinds of tara tannins with the content of 60%have significant differences(P<0.05),and the tannin contents measured by hide powder method were all higher than those measured by UV spectrophotometry,where the difference was about 1.5%-2.0%.And the correction constant i.e.,p=1.03,in the formula for determining the taratannin content by UV method was calculated.The results of UVVis absorption spectrum and high performance liquid chromatography analysis showed that the maximum absorption peaks of tara tannin and gallic tannin were both at 276 nm.At the same mass concentration,the absorption peak intensity of tara tannin was higher than that of gallic tannin.The peaking time of tara tannin was mainly in 20-40 min,and the peaking time of gallic tannin was mainly in 30-45 min.