文献类型：期刊文献

中文题名：超声波提取核桃内种皮多酚的响应面优化及其抗氧化研究

英文题名：Optimization of Extraction of Phenolics from Walnut Pellicle Using Response Surface Methodology and Their Antioxidant Capacities

作者：周晔[1,2,3,4,5] 王伟[1] 陶冉[2,3,4,5] 陈虹霞[2,3,4,5] 王成章[2,3,4,5] 裴东[1]

第一作者：周晔

通信作者：Pei, D.|[a0005c81e28dc32effca6]裴东;

机构：[1]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室;[2]中国林业科学研究院林产化学工业研究所;[3]生物质化学利用国家工程实验室;[4]国家林业局林产化学工程重点开放性实验室;[5]江苏省生物质能源与材料重点实验室

年份：2013

卷号：33

期号：4

起止页码：73-78

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;EI(收录号：20134216861054);Scopus(收录号：2-s2.0-84885334280);北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：'十二五'国家科技支撑计划资助(2013BAD14B01)

语种：中文

中文关键词：核桃内种皮;酚类物质;响应面法;抗氧化

外文关键词：walnut pellicle ; phenolics ; response surface methodology; antioxidant

分类号：TQ35

摘要：以核桃内种皮酚类物质提取得率为响应值,设计提取温度、提取时间以及液固比3因素3水平的Box-Behnken试验,采用响应面法确定超声波提取核桃内种皮酚类物质的最佳工艺,同时通过自由基清除能力和还原力试验,评价内种皮酚类物质的抗氧化性能。结果表明:0.1 g核桃内种皮粉末在固定超声波功率为100 W下的最佳提取工艺为提取温度53℃,提取时间55 min,液固比58∶1(mL∶g),总多酚得率为30.79%。相同浓度下的核桃内种皮酚类物质抗氧化性明显高于天然抗氧化剂VC,其清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基的半数抑制浓度(IC50)值为3.38 mg/L,小于Vc的4.72 mg/L;清除2,2'-联氮-双(3-乙基苯并噻唑-6-磺酸)(ABTS)自由基的IC50值为36.44 mg/L,小于Vc的82.23 mg/L。
Using temperature, time and liquid/solid ratio as independent variables, a Box-Behnken design was applied to optimise the extraction of phenolics from walnut pellicle by using response surface methodology. The results indicated that when the ultra- sonic power was set at 100 W , the optimum ultrasonic extraction conditions for walnut pellicle phenolics were as follows： extrac- tion temperature 53~C ,extraction time 55 rain, liquid/solid ratio 58 ： 1 （ mL ： g）. Under this optimum condition, the extraction yield was 30. 79%. It was found that the phenolics extracted from walnut pellicle behaved stronger antioxidant activities than ascorbic acid when they were under the same concentration. The ICs0 values of scavenging activity of pellicle phenolics on DPPH free radicals and ABTS were 3.38 mg/L and 36.44 mg/L, respectively, while those of ascorbic acid were 4.72 mg/L and 82.23 mg/L, respectively.