文献类型：期刊文献

英文题名：The genome of Populus alba x Populus tremula var. glandulosa clone 84K

作者：Qiu, Deyou[1] Bai, Shenglong[2] Ma, Jianchao[2] Zhang, Lisha[1] Shao, Fenjuan[1] Zhang, Kaikai[1,3] Yang, Yanfang[1] Sun, Ting[2] Huang, Jinling[2] Zhou, Yun[2] Galbraith, David W.[2,4,5] Wang, Zhaoshan[1] Sun, Guiling[2]

第一作者：邱德有

通信作者：Qiu, DY[1];Sun, GL[2]|[a000598d4c276c5f9aea6]邱德有;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Natl Forestry & Grassland Adm, Res Inst Forestry,Key Lab Tree Breeding & Cultiva, Beijing 100091, Peoples R China;[2]Henan Univ, Sch Life Sci, State Key Lab Cotton Biol, Key Lab Plant Stress Biol, Kaifeng 475004, Peoples R China;[3]Agr Univ Hebei, Coll Hort, Baoding 071001, Peoples R China;[4]Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA;[5]Univ Arizona, Bio5 Inst, Tucson, AZ 85721 USA

年份：2019

卷号：26

期号：5

起止页码：423-431

外文期刊名：DNA RESEARCH

收录：;Scopus(收录号：2-s2.0-85073580149);WOS:【SCI-EXPANDED(收录号:WOS:000509391000005)】；

基金：This project was supported by the special fund from Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration (ZDRIF201705), the National Natural Science Foundation of China (31771414), and the Program for Science and Technology Innovation Talents in Universities of Henan Province (18HASTIT041).

语种：英文

外文关键词：poplar 84K; genome sequencing; subgenome assignment; P. alba; P. tremula

摘要：Poplar 84K (Populus alba x P. tremula var. glandulosa) is a fast-growing poplar hybrid. Originated in South Korea, this hybrid has been extensively cultivated in northern China. Due to the economic and ecological importance of this hybrid and high transformability, we now report the de novo sequencing and assembly of a male individual of poplar 84K using PacBio and Hi-C technologies. The final reference nuclear genome (747.5 Mb) has a contig N50 size of 1.99 Mb and a scaffold N50 size of 19.6 Mb. Complete chloroplast and mitochondrial genomes were also assembled from the sequencing data. Based on similarities to the genomes of P. alba var. pyramidalis and P. tremula, we were able to identify two subgenomes, representing 356 Mb from P. alba (subgenome A) and 354 Mb from P. tremula var. glandulosa (subgenome G). The phased assembly allowed us to detect the transcriptional bias between the two subgenomes, and we found that the subgenome from P. tremula displayed dominant expression in both 84K and another widely used hybrid, P. tremula x P. alba. This high-quality poplar 84K genome will be a valuable resource for poplar breeding and for molecular biology studies.