文献类型：期刊文献

英文题名：Selection and validation of reference genes for quantitative gene expression normalization in Taxus spp.

作者：Zhang, Kaikai[1,2] Fan, Wei[3] Chen, Duanfen[2] Jiang, Luyuan[1,2] Li, Yunfeng[4] Yao, Zhiwang[2] Yang, Yanfang[1] Qiu, Deyou[1]

第一作者：Zhang, Kaikai

通信作者：Yang, YF[1];Qiu, DY[1]|[a000598d4c276c5f9aea6]邱德有;

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing 100091, Peoples R China;[2]Agr Univ Hebei, Coll Hort, Baoding 071001, Peoples R China;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[4]South China Agr Univ, Coll Agr, Guangzhou 510642, Peoples R China

年份：2020

卷号：10

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000603258300014)】；

基金：We are thankful to Professor Z.W. Huang for his helpful in statistical analysis. This work was supported by a Grant for National non-profit Research Institutions of Chinese Academy of Forestry (CAFYBB2018SY009), National Natural Science Foundation of China (31570675), and the projects of the National Key Research and Development Program of China (2017YFD060070605).

语种：英文

摘要：Quantitative real-time PCR (qRT-PCR) is commonly used to measure gene expression to further explore gene function, while suitable reference genes must be stably expressed under different experimental conditions to obtain accurate and reproducible data for relative quantification. Taxol or paclitaxel is an important anticancer compound mainly identified in Taxus spp. The molecular mechanism of the regulation of taxol biosynthesis is current research goal. However, in the case of Taxus spp., few reports were published on screening suitable reference genes as internal controls for qRT-PCR. Here, eight reference genes were selected as candidate reference genes for further study. Common statistical algorithms geNorm, NormFinder, BestKeeper, Delta Ct, and RefFinder were used to analyze the data from samples collected from a cell line of Taxusxmedia under various experimental conditions and from tissues of Taxus chinensis var. mairei. The expression patterns of TcMYC under salicylic acid treatment differed significantly, with the best and worst reference genes in the cell line. This study screened out suitable reference genes (GAPDH1 and SAND) under different treatments and tissues for the accurate and reliable normalization of the qRT-PCR expression data of Taxus spp. At the same time, this study will aid future research on taxol biosynthesis-related genes expression in Taxus spp., and can also be directly used to other related species.