文献类型：期刊文献

中文题名：青龙衣中胡桃醌的提取及其抑菌活性研究

英文题名：Extraction and Antimicrobial Activity of Juglone from Pericarpium Juglandis

作者：李文君[1] 王成章[1] 刘辰婧[2]

第一作者：李文君

机构：[1]中国林业科学研究院林产化学工业研究所,生物质化学利用国家工程实验室,国家林业和草原局林产化学工程重点实验室,江苏省生物质能源与材料重点实验室,江苏南京210042;[2]南京林业大学轻工与食品学院,江苏南京210037

年份：2019

卷号：39

期号：5

起止页码：95-100

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：江苏省生物质能源与材料重点实验室基本科研业务费项目(JSBEM-S-201810);中国林科院中央级公益性科研院所基本科研业务费专项资金(CAFYBB2017ZA004-2)

语种：中文

中文关键词：青龙衣;胡桃醌;超声波辅助;纳米银溶液;抑菌活性

外文关键词：pericarpium juglandis;nutcrone;ultrasound assisted;nano-silver solution;antibacterial activity

分类号：TQ35

摘要：以青龙衣为原料,采用超声波辅助提取青龙衣中胡桃醌,通过单因素和正交试验优化得到最佳提取工艺条件;并以甲醇和商用YZN-Y500纳米银抗菌溶液作对照,考察最佳提取工艺条件下得到的质量浓度分别为0.20、0.02、0.01、0.005和0.0025g/L的青龙衣提取物样品溶液,对枯草芽孢杆菌、肺炎链球菌、金黄色葡萄球菌和表皮葡萄球菌的抑菌活性。研究结果表明:以甲醇为浸提溶剂,浸泡时间6h,液料比5∶1(mL∶g),超声波作用时间35min的最佳提取条件下胡桃醌得率0.246%,浸膏得率13.88%。青龙衣提取物溶液具有较强的抑菌活性,且溶液浓度越高,抑菌效果越明显;质量浓度为0.20、0.02g/L的青龙衣提取物样品溶液对4种菌的抑菌圈直径可达15~25mm,抑菌效果强于0.01g/L的商用纳米银溶液。
Juglone was extracted from pericarpium juglandis by ultrasound-assisted method. The optimal conditions of extracting juglone were obtained by single experiment and orthogonal test as follows: soaking time was 6 h using methanol as solvent, ratio of liquid to material was 5∶1(mL∶ g), ultrasonic time was 35 min, and the yield of juglone was 0.246% and the yield of extract was 13. 88%. The antibacterial activities of extract on Bacillus subtilis, Streptococcus pneumoniae, Staphylococcus aureus and S. epidermidis of juglone was investigated by mass concentration of 0. 002 5 - 0. 20 g/ L, compared with commercial YZN-Y500 nano-silver. The results showed that the optimum extraction conditions was as follows: The antibacterial effect of pericarpium juglandis extract on the mass concentrations of 0.20g/ L and 0. 02 g/ L was stronger than that of commercial silver nanoparticles.