文献类型：期刊文献

中文题名：短毛切梢小蠹微卫星富集文库的构建与分析

英文题名：Construction and Analysis of Enriched Microsatellite Library of Tomicus brevipilosus

作者：曹荟[1] 朱家颖[1] 杨璞[2] 赵宁[1] 杨斌[1]

第一作者：曹荟

机构：[1]西南林业大学云南省森林灾害预警与控制重点实验室;[2]中国林业科学研究院资源昆虫研究所

年份：2012

卷号：32

期号：5

起止页码：62-65

中文期刊名：西南林业大学学报：自然科学

收录：CSTPCD

基金：西南林业大学科学研究基金面上项目(111128)资助;林业公益性行业科研专项(201004067)资助

语种：中文

中文关键词：短毛切梢小蠹;微卫星;文库构建;序列分析

外文关键词：Tomicus brevipilosus ; microsatellite ; library construction; sequence analysis

分类号：S718.7

摘要：采用生物素-磁珠富集法,构建短毛切梢小蠹微卫星富集文库。在该文库中随机挑选100个克隆作测序分析,结果表明:具微卫星位点的克隆占91%;具有10次以上碱基重复次数的微卫星序列占36%,其中最高碱基重复次数为63次;在这些微卫星序列中,完美型占47.3%,非完美型占24.2%,混合型占16.5%。表明获得的微卫星文库是高质量的文库,完美型的比例高于不完美型和混合型,与真核生物微卫星序列特征相符。
The enriched microsatellite library of Tomicus brevipilosus （Eggers） was constructed using biotin-magnetic bead enrichment method. One hundred clones randomly chosen from the library were sequenced. The results showed that 91% of microsateUite loci were obtained. Among which the microsatellite sequences with more than 10 times of alkaline base repetitions accounted for 36%, and the highest alkaline base repetition was 63 times. From these microsatellites, 47.3% （about 43 repeat motifs） were perfect, 24.2% （about 22 repeat motifs） were imperfect, and 16.5% （about 15 repeat motifs） were compound. The results indicated that the enriched microsatellite library was of high-quality, in which the proportion of perfect microsatellites types was higher than that of the imperfect and compound types, which was in accordance with the feature of microsatellite sequences of other eukaryote.