文献类型：期刊文献

英文题名：PsPRE1 is a basic helix-loop-helix transcription factor that confers enhanced root growth and tolerance to salt stress in poplar

作者：Du, Jiujun[1] Ge, Xiaolan[1] Wei, Hantian[1] Zhang, Min[1] Bai, Yongxia[1] Zhang, Lei[1,2] Hu, Jianjun[1,2]

第一作者：Du, Jiujun

通信作者：Zhang, L[1];Hu, JJ[1];Zhang, L[2];Hu, JJ[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry Southe, Nanjing 210037, Peoples R China

年份：2023

卷号：3

外文期刊名：FORESTRY RESEARCH

收录：Scopus(收录号：2-s2.0-85175307601);WOS:【ESCI(收录号:WOS:001380005800003)】；

基金：Acknowledgments This study was supported by the Major Project of Agricul-tural Biological Breeding (2022ZD0401501) , the National Key Re-search and Development Program of China (2021YFD2200201) and the National Natural Science Foundation (32071797) . We would like to thank A&L Scientific Editing ( www.alpublish.com ) for its linguistic assistance during the preparation of this manuscript.

语种：英文

摘要：The basic helix-loop-helix (bHLH) family of transcription factors is one of the largest and oldest transcription factor families in plants. Members of the bHLH family regulate various growth and metabolic processes in plants. We used quantitative trait locus (QTL) mapping and transcriptome sequencing (RNA-seq) to identify PRE1 as a candidate bHLH transcription factor associated with root dry weight (RDW) in poplar. PRE1 was highly expressed in the roots and xylem, and was responsive to gibberellin, salicylic acid, drought, and salt stress. We cloned the PRE1 homolog from Populus simonii 'Tongliao1', referred to as PsPRE1, and transformed it into 84K poplar ( Populus alba x Populus glandulosa). The overexpression of PsPRE1 in 84K poplar increased adventitious root development, fresh weight, total root number, and maximum root length. Poplar lines overexpressing PsPRE1 also exhibited enhanced salt tolerance while retaining a normal growth phenotype in the presence of salt stress. Catalase (CAT) activity in the PsPRE1 overexpression lines was higher than that of the wild-type, which may play a role in detoxifying stress-induced hydrogen peroxide production. An RNA-seq analysis of the PsPRE1 overexpression line revealed several differentially expressed genes (DEGs) involved in or related to auxin-, gibberellin-, and salicylic acid pathways, which indicates that the regulation of root development in poplar by PsPRE1 may involve multiple hormones.