文献类型：期刊文献

中文题名：银杏叶聚戊烯醇对小鼠免疫功能和诱导肿瘤细胞凋亡的研究

英文题名：STUDIES OF GINKGO BILOBA L. POLYPRENOLS ON IMMUNOLOGICAL FUNCTION AND INDUCING APOPTOSIS OF TUMORS IN MOUSE

作者：王成章[1] 沈兆邦[1] 刘妤婵[1] 高凌[2]

第一作者：王成章

机构：[1]中国林业科学研究院林产化学工业研究所;[2]江苏省肿瘤防治研究所

年份：2005

卷号：25

期号：3

起止页码：9-12

中文期刊名：林产化学与工业

外文期刊名：Chemistry & Industry of Forest Products

收录：CSTPCD;;Scopus;CSCD:【CSCD_E2011_2012】；

基金：国家自然科学基金资助项目(C02060103);国家林业局948项目资助(2003436)

语种：中文

中文关键词：银杏叶;聚戊烯醇;巨噬细胞;吞噬功能;细胞凋亡

外文关键词：Ginkgo biloba L. leaves; polyprenols ; maerophages ; phagocytic function ; apoptosis

分类号：TQ91;S792.95

摘要：用不同剂量银杏叶聚戊烯醇(GP)给正常小鼠和荷瘤小鼠灌胃,用碳粒廓清法分析正常小鼠巨噬细胞吞噬功能;采用流式细胞术(FCM)检测S180荷瘤小鼠的凋亡细胞比率(APO)、S期细胞比率(SPF)和增殖指数(PI)的变化,分析T细胞亚群CD4/CD8比值的影响。结果表明,10和20mg/kg的GP能提高正常小鼠巨噬细胞的吞噬功能,对碳粒的清除作用高于环磷酰胺(CTX);高剂量组(40mg/kg)GP与对照组比较,能增加肝癌(Heps)荷瘤小鼠胸腺指数和艾氏腹水癌(EC)荷瘤小鼠脾指数。5mg/kgGP对S180荷瘤小鼠细胞的APO为6.35,明显高于阴性对照组及其它给药组,使S180荷瘤小鼠CD4/CD8比值接近正常小鼠。
Different dosages of polyprenols from Ginkgo biloba L. leaves（GP） were perfused to normal mouse and tumor mouse through stomach respectively. The phagocytic function of the macrophages were measured in the normal mouse by engulfing carbon granula ability. Apoptosis （APO）, S phase fraction （SPF）, proliferation index（PI） and CD4/CD8 rate of T lymphocytes were investigated by flow cytometry （FCM） in mouse with S180 tumor. The results showed that polyprenols of G. biloba L. at 10 and 20 mg/kg could increase remarkably the phagocytic function which was higher than the clearance of cyclophosphamide （CTX）. In comparison with the control group, the high GP group （40 mg/kg） could increase the index of the thymus gland in mouse with heps and index of the spleen in mouse with EC. The APO level in mouse with S180 tumor in 5 mg/kg GP was 6.35 ,which went up significantly compared to the control group and other administration groups, and made the rate of CD4/CD8 near to that of the normal mouse.