文献类型：期刊文献

中文题名：曼地亚红豆杉NAC基因家族鉴定及表达分析

英文题名：Identification and Expression Analysis of NAC Gene Family in Taxus×media Rehder

作者：张恺恺[1] 杨立莹[3] 丰美静[1,2] 张林凤[1] 陈段芬[2] 邱德有[1] 杨艳芳[1]

第一作者：张恺恺

机构：[1]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,国家林业和草原局林木培育重点实验室,北京100091;[2]河北农业大学园艺学院,河北保定071001;[3]中国花卉协会,北京100020

年份：2022

卷号：35

期号：2

起止页码：97-103

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：“十三五”国家重点研发计划子课题“红豆杉高效培育技术研究”(2017YFD060070605);国家林业和草原局科技发展中心项目(KJZXSA202037);国家自然科学基金项目(31570675)项目资助。

语种：中文

中文关键词：红豆杉;NAC基因;基因鉴定;表达分析

外文关键词：Taxus spp.;NAC gene;gene identification;expression analysis

分类号：S718.46

摘要：[目的]筛选红豆杉NAC转录因子家族中影响根系形成的关键基因,探索红豆杉根系生长发育分子机理。[方法]利用曼地亚红豆杉全长转录组数据,通过生物信息学方法鉴定NAC转录因子,并对筛选出的NAC转录因子进行蛋白结构及基因组织表达谱等分析。[结果]共鉴定出44个NAC转录因子,其在N端均具有典型的保守NAC结构域,分别聚类到拟南芥的7个亚家族中,蛋白三级结构较为相似,且成员大多含有5个保守亚结构域。组织表达谱显示TmNAC15、16、18、21、22、29、39、40、41和44在根中表达水平高于茎和叶。[结论]从曼地亚红豆杉中共鉴定出44个NAC转录因子,其结构较为保守,且聚类为7个亚家族,其中成员TmNAC21、22、39、40和44极有可能参与红豆杉根系生长发育。
[Objective]To obtain the key NAC genes involving in regulating the root growth and development of yew trees.[Method]Basing on the full-length transcriptome data of Taxus×media,bioinformatics methods were used to identify NAC transcription factors.The protein structure and gene tissue expression profile of the selected NAC genes were also analyzed.[Result]A total of 44 NAC transcription factors were identified,which have typical conserved NAC domains at the N-terminal,and most of the members contain 5 conserved structural functional subdomains.Phylogenetic tree analysis showed that 44 T.×media NAC(TmNAC)proteins were clustered into 7 subfamilies of Arabidopsis.Sequence structure analysis indicated that the tertiary structures of the TmNAC proteins were similar,but the structures of the representative member of different subfamily also showed obvious differences.The expression results showed that TmNAC15,16,18,21,22,29,39,40,41,and 44 had higher expression level in roots than stems and needles.[Conclusion]In this study,44 TmNAC transcription factors were identified from T.×media and they were clustered into 7 subfamilies.Among them,TmNAC21,22,39,40,and 44 may be involved in the root growth and development progresses of yew trees.