文献类型：期刊文献

中文题名：产没食子酸脱羧酶的菌种筛选及其酶活研究

英文题名：Screen the strains of producing gallic acid decarboxylase and enzymic activities

作者：李文君[1,2,3,4] 王成章[1,2,3,4] 张昌伟[1,2,3,4] 叶建中[1,2,3,4]

第一作者：李文君

机构：[1]中国林业科学研究院林产化学工业研究所;[2]生物质化学利用国家工程实验室;[3]国家林业局林产化学工程重点开放性实验室;[4]江苏省生物质能源与材料重点实验室

年份：2016

卷号：44

期号：12

起止页码：1-6

中文期刊名：化学工程

外文期刊名：Chemical Engineering(China)

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家"863"项目(2014AA021802);国际合作项目(中俄项目2014DFR31300)

语种：中文

中文关键词：没食子酸脱羧酶;微生物降解;焦性没食子酸;酶学性质

外文关键词：gallic acid decarboxylase; microbial degradation; pyrogallic acid; enzymatic properties

分类号：Q814.1

摘要：为了筛选产没食子酸脱羧酶的菌种并对其相应的酶活性质进行研究,文中首先利用底物诱导对可以产没食子酸脱羧酶的不同菌种进行了筛选,初步研究了没食子酸脱羧酶的专一性及影响其酶活的因素,由结果可知:待筛选的5株菌种均能降解没食子酸生成焦性没食子酸,其降解效果由强到弱为弗式柠檬酸杆菌(Citrobacter freandii)CICC 22001、阴沟肠杆菌(Enterobacter cloacae)CICC 10017、肠杆菌属(Enterobacter spp.)CICC 23468、阴沟肠杆菌(Enterobacter cloacae)CICC 20772、阴沟肠杆菌(Enterobacter cloacae)CICC 20804;没食子酸脱羧酶(GAD)对没食子酸具有较强的专一性,在粗酶液5.0 m L,没食子酸0.4 mg/m L,发酵温度35℃,磷酸盐缓冲溶液pH=6.0对其酶活的促进作用最强,而金属离子的促进作用强弱为Mg^(2+)>Sn^(2+)>Cu^(2+)>Zn^(2+)>K+>Ca^(2+)>Fe^(2+),添加剂的抑制作用强弱为EDTA>SDS>Triton X-100>Tween 20。
In order to screen the strains to produce the gallic acid decarbolxylase and study of their enzymic activities, the different bacterial strains with the abilities of degrading gallic acid were screened by the substrate induction test, and the enzymatic specificity and properties were studied preliminarily. The results show that all of 5 screening strains can degrade gallic acid to pyrogallol, and their effect from strong to weak is Citrobacterfreandii CICC 22001, Enterobacter cloacae CICC 10017, Enterobacter spp. CICC 23468, Enterobacter cloacae CICC 20772, Enterobacter cloacae CICC 20804; GAD has strong enzymatic specificity in degrading gallic acid. Phosphate buffer solution has the strongest promoting effect at 5. 0 mL GAD, 0. 4 mg/mL gallic acid, 35℃ fermentation temperature, and pH 6.0. The effect of metal ions on GAD from strong to weak is Mg2＋ 〉 Sn2 ＋ 〉 Cu2＋ 〉 Zn2＋ 〉 K＋ 〉 Ca2＋ 〉 Fe2＋ , and the effect of the inhibition additives from strong to weak is EDTA 〉 SDS 〉 Triton X-100 〉 Tween 20.