文献类型：期刊文献

英文题名：De novo sequencing and a comprehensive analysis of purple sweet potato (Impomoea batatas L.) transcriptome

作者：Xie, Fuliang[1] Burklew, Caitlin E.[1] Yang, Yanfang[2,3] Liu, Min[4] Xiao, Peng[5] Zhang, Baohong[1] Qiu, Deyou[2,3]

第一作者：Xie, Fuliang

通信作者：Zhang, BH[1]

机构：[1]E Carolina Univ, Dept Biol, Greenville, NC 27858 USA;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[3]State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[4]China Agr Univ, Coll Plant Sci, Beijing 102206, Peoples R China;[5]E Carolina Univ, Dept Math, Greenville, NC 27858 USA

年份：2012

卷号：236

期号：1

起止页码：101-113

外文期刊名：PLANTA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000305682100008)】；

语种：英文

外文关键词：Anthocyanin; Gene expression; Microsatellite markers; RNA-sequencing; Simple sequence repeat (SSR); Sweet potato; Transcriptome

摘要：High-throughput RNA sequencing was performed for comprehensively analyzing the transcriptome of the purple sweet potato. A total of 58,800 unigenes were obtained and ranged from 200 nt to 10,380 nt with an average length of 476 nt. The average expression of one unigene was 34 reads per kb per million reads (RPKM) with a maximum expression of 1,935 RPKM. At least 40,280 (68.5%) unigenes were identified to be protein-coding genes, in which 11,978 and 5,184 genes were homologous to Arabidopsis and rice proteins, respectively. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that 19,707 (33.5%) unigenes were classified to 1,807 terms of GO including molecular functions, biological processes, and cellular components and 9,970 (17.0%) unigenes were enriched to 11,119 KEGG pathways. We found that at least 3,553 genes may be involved in the biosynthesis pathways of starch, alkaloids, anthocyanin pigments, and vitamins. Additionally, 851 potential simple sequence repeats (SSRs) were identified in all unigenes. Transcriptome sequencing on tuberous roots of the sweet potato yielded substantial transcriptional sequences and potentially useful SSR markers which provide an important data source for sweet potato research. Comparison of two RNA-sequence datasets from the purple and the yellow sweet potato showed that UDP-glucose-flavonoid 3-O-glucosyltransferase was one of the key enzymes in the pathway of anthocyanin biosynthesis and that anthocyanin-3-glucoside might be one of the major components for anthocyanin pigments in the purple sweet potato. This study contributes to the molecular mechanisms of sweet potato development and metabolism and therefore that increases the potential utilization of the sweet potato in food nutrition and pharmacy.