文献类型：期刊文献

中文题名：倍蚜种间亲缘关系及角倍蚜种群分化的RAPD分析

英文题名：RAPD Analysis of Phylogenetic Relationships in Chinese Gallnut Aphids (Homoptera: Pemphigidae) and Genetic Differentiation in Four Populations of Schlechtendalia chinensis

作者：杨子祥[1] 陈晓鸣[1] 冯颖[1] 张燕平[1]

第一作者：杨子祥

机构：[1]中国林业科学研究院资源昆虫研究所国家林业局资源昆虫培育与利用重点实验室

年份：2007

卷号：43

期号：7

起止页码：44-50

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家科技部基础性研究项目(2000DEB100035)资助。

语种：中文

中文关键词：倍蚜;随机扩增多态性DNA;亲缘关系;遗传距离;聚类分析

外文关键词：Chinese gallnut aphids ; RAPD ; phylogenetic relationship ; Nei＇ s genetic distance ; cluster analysis

分类号：S718.7

摘要：从40条RAPD随机引物中筛选了13条和9条,分别对11种倍蚜和角倍蚜4个地理种群的DNA进行PCR扩增和分析。结果表明:倍蚜的遗传距离在不同属之间为0.4828±0.1708,不同种之间为0.2520±0.1780,不同亚种之间为0.1472±0.0764,聚类分析反映了倍蚜属间、种间的亲缘关系及其远近程度,与形态分类结果基本一致;圆角倍蚜属与其他3个属的差异明显,可能是倍蚜中较早分化的类群。角倍蚜不同种群间的遗传距离为0.0759±0.0302,种群间具有丰富的DNA序列多态性并出现了一定程度的遗传分化,造成这种分化的原因可能是地理上的隔离。
The RAPD technique was used to determine phylogenetic relationships of Chinese gallnut aphids and genetic differentiation in four populations of Schlechtendalia chirtensis, a major Chinese gallnut aphid. 13 primers and 9 primers were selected from 40 primers and used to amplify their genome DNA. The genetic distances between genera, species and subspecies are 0.482 8 ± 0. 170 8,0.252 0 ± 0. 178 0,0. 147 2 ± 0.076 4, respectively. Dendrogram based on Nei＇s genetic distance displayed the phylogenetic relationships of different taxa and almost coincided with morphological taxonomy. Our result suggested that there is a distinct difference between the Nurudea and other three genera. It may be implied that the Nurudea was the first differentiation group among Chinese gall aphids. The genetic distance among 4 populations of S. chirtensis is 0.075 9 ± 0.030 2. A certain extent genetic variation among the populations had emerged and it maybe caused by the geographical isolation.