文献类型：期刊文献

中文题名：不同碳链长度的没食子酸酯对小鼠皮肤伤口愈合的影响

英文题名：Effect of Gallates With Different Length of Carbon Chain on Skin Wound Healing in Mice

作者：李想[1,2] 张弘[2] 杨帅飞[1] 周备[1] 刘兰香[2]

第一作者：李想

机构：[1]西南林业大学生命科学学院,云南昆明650224;[2]中国林业科学研究院高原林业研究所,国家林业和草原局特色森林资源工程技术研究中心,国家林业和草原局资源昆虫培育与利用重点实验室,云南昆明650233

年份：2024

卷号：44

期号：6

起止页码：151-159

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;Scopus;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：国家重点研发计划资助项目(2022YFD2200803)。

语种：中文

中文关键词：没食子酸酯;伤口愈合;生长因子;碳链长度;损伤模型

外文关键词：gallate;wound healing;growth factor;carbon chain length;injury model

分类号：TQ35

摘要：为探究不同碳链长度的没食子酸酯对皮肤伤口愈合的作用,采用小鼠背部全皮肤机械损伤模型,选取代表性短链没食子酸丙酯(GA-C3)、长链没食子酸十八烷基酯(GA-C18)以及超长碳链的没食子酸二十八烷基酯(GA-C28)为实验药物对伤口进行治疗,同时设置模型组和空白组,通过测定伤口愈合率、观察组织切片,以及检测白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肝细胞生长因子(HGF)、成纤维细胞生长因子-β(FGF-β)、转化生长因子-β1(TGF-β1)、血管内皮细胞生长因子(VEGF)、信号转导子与转录激活子3(STAT3)和非受体酪氨酸激酶(Src)等,考察各没食子酸酯等对伤口的促愈效果。研究结果表明:在创伤处使用不同的没食子酸酯均可在前7天促进小鼠伤口愈合,其中长碳链的化合物GA-C18的效果稍好于短碳链GA-C3和超长碳链GA-C28,但3者之间没有显著性差异;在第14天,3个药物组的小鼠伤口愈合率都在98.5%以上,均显著好于模型组(94.3%)。组织切片显示,没食子酸酯治疗的创伤组织在第3天炎症反应小,在第7天显示出比模型组更丰富的肉芽组织、纤维细胞、新生血管等。此外,检测结果显示:没食子酸酯用于创伤处可在3天和7天内刺激动物机体分泌更多的因子如IL-1β、IL-6、HGF、FGF-β、TGF-β1、VEGF、STAT3和Src等,从而加速伤口愈合的炎症阶段向增殖、重塑阶段转换,减少炎症反应和促进伤口愈合。
In order to investigate the effect of gallates with different lengths of carbon chain on skin wound healing,the mechanical injury model of the whole skin on the back of mouse was adopted,and the representative short-chain propyl gallate(GA-C3),long-chain gallate octadecyl ester(GA-C18)and ultra-long carbon chain gallate octacosyl ester(GA-C28)were selected as experimental drugs to treat the wound,and both model and blank groups were set up.The effect of gallates on the wound healing was investigated by measuring the rate of wound healing,observing tissue sections,detecting interleukin-1β(IL-1β),interleukin-6(IL-6),hepatocyte growth factor(HGF),fibroblast growth factor-β(FGF-β),transforming growth factor-β1(TGF-β1),vascular endothelial growth factor(VEGF),signal transducer and activator of transcription 3(STAT3),Src protein(Src)and so on.The results showed that the use of different gallates at the wound site could promote wound healing on mouse in the first 7 days.GA-C18 with a long carbon chain had a slightly better effect than short carbon chain GA-C3 and super long carbon chain GA-C28,but there was no significant difference between the above three.On day 14,the wound healing rate of the mice in three drugs groups was more than 98.5%,which was significantly better than that of the model group(94.3%).Tissue sections showed that the gallate-treated wound tissue had a low inflammatory response on day 3 and more abundant granulation tissue,fibrocytes and neovasculature on day 7 than the model group.In addition,the cytokine detection results showed that the gallates used in the wound could stimulate the animal body to secrete more factors such as IL-1β,IL-6,HGF,FGF-β,TGF-β1,VEGF,STAT3 and Src within 3 and 7 days,thereby accelerating the transition from the inflammatory stage of wound healing to the proliferative and remodelling stage,reducing the inflammatory response and promoting wound healing.