文献类型：期刊文献

中文题名：木豆叶蛋白提取工艺的初步研究

英文题名：The Preliminary Technological Research on Extraction of Leaf Protein Concentrate from Cajanus cauan

作者：张建云[1] 邱坚[2] 李正红[1] 刘秀贤[1] 惠雅玲[1] 周朝鸿[1]

第一作者：张建云

机构：[1]中国林业科学研究院资源昆虫研究所;[2]西南林学院资源学院

年份：2001

卷号：21

期号：1

起止页码：40-44

中文期刊名：西南林学院学报

外文期刊名：Journal of Southwest Forestry College

基金：国家林业局"948"引进项目! (98- 4- 15 );云南省应用基础研究基金资助项目! (97C0 34Q)

语种：中文

中文关键词：木豆;提取工艺;正交设计;叶蛋白;加热性;酸法;碱法;饲料蛋白

外文关键词：Cajanus cauan ;Extraction technology;Orthogonal design;Leaf protein concentrate(LPC)

分类号：S816.35;S816.4

摘要：采用正交设计对配合使用酸、碱以及加热法提取木豆叶蛋白工艺及其工艺条件进行了初步研究 ,结果表明 :木豆叶蛋白提取工艺条件为 :打浆时间 1min ,絮凝温度 80℃ ,pH值 1 0 ,料液比 1∶3;该工艺提取叶蛋白的得率为 5 1 1 % ,其蛋白质含量为 33 37% ,原料总提取率为 6 4 0 6 % .
The optimum technique and conditions of extracting Leaf Protein Concentrate(LPC)from the fresh leaves of Cajanus cauan were studied in this paper,and three methods of precipitating leaf protein with acid,alkali and heat,and the orthogonal design were utilized in this technique.The results showed that the most optimum extracting conditions included the normal temperature,the ratio of 1∶3 for fresh leaf to water,the pH of 10,the flocculating temperature of 80℃and 1 minute for the beatig times.By using this technique,about 5.11g of LPC containing about 33.37% of real protein were extracted from 100g fresh leaves and about 64.06% of total nitrogen in fresh leaf of Cajanus cauan was retrieved.