文献类型：期刊文献

英文题名：Green Tea Epigallocatechin-3-gallate Ameliorates Lipid Accumulation and Obesity-Associated Metabolic Syndrome via Regulating Autophagy and Lipolysis in Preadipocytes and Adipose Tissue

作者：Peng, He[1] Cheng, Qi[1] Chen, Jiajun[1] Wang, Ying[2] Du, Menghao[3] Lin, Xiaojian[1] Zhao, Qian[1] Chen, Shengjia[1] Zhang, Jingsa[1] Wang, Xingya[1,4,5]

第一作者：Peng, He

通信作者：Wang, XY[1];Wang, XY[2];Wang, XY[3]

机构：[1]Zhejiang Chinese Med Univ, Sch Pharmaceut Sci, Hangzhou 311400, Peoples R China;[2]Zhejiang A&F Univ, Coll Food & Hlth, Hangzhou 311300, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[4]Taizhou Univ, Sch Pharmaceut Sci, Taizhou 318000, Peoples R China;[5]Taizhou Univ, Inst Adv Studies, Taizhou 318000, Peoples R China

年份：2025

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20252018408197);Scopus(收录号：2-s2.0-105004752337);WOS:【SCI-EXPANDED(收录号:WOS:001485357300001)】；

基金：This research was funded by "The Pioneer" and "Leading Goose" R&D Program of Zhejiang (2023C02045).

语种：英文

外文关键词：obesity; green tea; epigallocatechin-3-gallate(EGCG); autophagy; lipolysis; adipogenesis; lipogenesis

摘要：Previous studies have shown that epigallocatechin-3-gallate (EGCG), the most abundant catechin in green tea, demonstrates promising antiobesity effects. While autophagy mediates obesity via preadipocyte differentiation and lipogenesis, EGCG's potential autophagy-dependent antiobesity mechanism remains unclear. We used 3T3-L1 cells and high-fat-diet (HFD)-fed mice to examine how EGCG inhibits adipogenesis and lipogenesis via autophagy. EGCG (50 or 100 mg/kg) significantly attenuated HFD-induced weight gain, fat accumulation, hyperlipidemia, and glucose intolerance in mice. It also enhanced autophagy and lipolysis in white adipose tissue (WAT). EGCG profoundly inhibited terminal preadipocyte differentiation and lipid droplet formation in 3T3-L1 cells accompanied by reduced PPAR gamma, C/EBP alpha, and FASN expressions. Mechanistically, EGCG inhibited autophagy during the early stage of preadipocyte differentiation, as evidenced by increased autophagosome accumulation and impaired autophagic flux. The antiadipogenic effect of EGCG was further aggravated by the autophagy inhibitor chloroquine. Meanwhile, EGCG increased p38 and AMPK/ACC phosphorylation while inhibiting JNK phosphorylation in 3T3-L1 cells at an early stage of adipogenesis. Interestingly, EGCG reduced the expression of lipolytic enzymes HSL and ATGL, and it decreased glycerol contents in differentiated 3T3-L1 cells. These findings provide novel insights into the mechanism of using green tea EGCG in functional foods to combat obesity by targeting autophagy and lipolysis.