文献类型：期刊文献

中文题名：水竹叶总黄酮的提取及纯化工艺研究

英文题名：Studies on Extraction and Purification of Flavonoids from Phyllostachys heteroclata Oliver

作者：陈文英[1,2] 王成章[1,2] 叶建中[1,2] 陈虹霞[1,2]

第一作者：陈文英

机构：[1]中国林业科学研究院林产化学工业研究所;[2]国家林业局林产化学工程重点开放性实验室

年份：2007

卷号：27

期号：B10

起止页码：73-76

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;Scopus;CSCD:【CSCD_E2011_2012】；

基金：国家科技支撑计划项目资助(2006BAD19B0803)

语种：中文

中文关键词：水竹叶;总黄酮;正交试验;提取纯化

外文关键词：Phyllostachys heteroclata Oliver leaves ;flavonoids ;orthogonal test ;purification

分类号：TQ91

摘要：以水竹叶为原料,采用水浴和超声波两种提取方法,通过单因素与正交试验确定了水竹叶总黄酮提取的最佳条件,并筛选了不同树脂对总黄酮的吸附和解吸附特性。实验结果表明:水浴提取最佳条件为:温度50℃,提取时间2.0 h,乙醇体积分数60%,固液比1∶20(g∶mL,下同);总黄酮得率1.65%。影响得率的因素为:固液比>乙醇体积分数>提取温度>提取时间。超声波提取最佳条件为:2.0 g水竹叶,超声波作用时间30 min,乙醇体积分数60%,固液比1∶15;总黄酮得率为1.27%。影响得率的因素为:超声波作用时间>乙醇体积分数>固液比。AB-8树脂对总黄酮纯化具有较好效果,体积分数为70%的乙醇是较好的解吸剂。
Flavonoids from Phyllostachys heteroclata Oliver were extracted by methods of waterbath and ultrasonic extractions. The optimum extraction conditions were obtained by single factor and orthogonal tests, Different kinds of macro-reticular resins were tested for purification. The results showed that the yield of flavonoids was 1.65 % by waterbath extraction under the conditions of 60 % ethanol, 50℃, 1 ： 20 solid-liquid ratio and 1.5 h. Order of the factors which affected waterbath extraction was ： ratio of soild to liquid 〉 concentration of ethanol solution 〉 extraction temperature 〉 extraction time. The yield of flavonoids was 1,27 % by ultrasonic extraction under the conditions of 60 % ethanol, 1 ： 15 solid-liquid ratio and 30 min. Order of the factors which affected ultrasonic-assisted extraction was： extraction time 〉 volume fraction of ethanol solution 〉 ratio of soild to liquid. It was found that AB-8 macro-reticular resin was superior for the purification of flavonoids, and 70 % ethanol was a good eluant.