文献类型：期刊文献

中文题名：太行山地区南方红豆杉SSR遗传多样性分析

英文题名：Genetic Diversity of Taxus chinensis var.mairei in Taihang Mountains Based on SSR Molecular Markers

作者：杜雨晴[1] 蒋路园[1] 汪奕衡[2] 吴宸炜[1] 杨梦露[1] 刘旭升[3] 王晓君[4] 邱德有[1] 樊玮[5] 杨艳芳[1]

第一作者：杜雨晴

机构：[1]中国林业科学研究院林业研究所林木遗传育种全国重点实验室、国家林业和草原局林木培育重点实验室,北京100091;[2]道地药材品质保障与资源持续利用全国重点实验室、中国中医科学院中药资源中心,北京100700;[3]河南森涛林业科技有限公司,济源459000;[4]济源市科学院,济源459000;[5]中国林业科学研究院林木遗传育种全国重点实验室,北京100091

年份：2025

卷号：41

期号：12

起止页码：214-224

中文期刊名：生物技术通报

外文期刊名：Biotechnology Bulletin

收录：;北大核心:【北大核心2023】；

基金：国家林业和草原局科技发展中心生物安全与遗传资源管理项目(KJZXSA202037);国家自然科学基金面上项目(32371907)。

语种：中文

中文关键词：南方红豆杉;太行山地区;SSR标记;遗传多样性;遗传变异

外文关键词：Taxus chinensis var.mairei;Taihang Mountain;SSR marker;genetic diversity;genetic variation

分类号：S791.49

摘要：【目的】红豆杉(Taxus spp.)为国家一级濒危保护植物,阐明太行山地区南方红豆杉种群遗传多样性和遗传变异,可为南方红豆杉遗传多样性保护及培育新品种提供指导。【方法】基于前期筛选出的8对稳定性和多态性良好的SSR引物,对太行山区的9个南方红豆杉种群以及3个来自浙江、湖南和福建3省的南方红豆杉种群(共106个体)的遗传多样性和遗传变异进行分析。【结果】12个南方红豆杉种群的平均观测等位基因数(Na)为2.781,平均有效等位基因数(Ne)为2.074,Shannon多样性指数(I)为0.679,平均观测杂合度Ho=0.390,平均期望杂合度He=0.381。通过对种群间遗传分化指数(Fst)和基因流强度(Nm)进行分析发现,太行山区南方红豆杉种群间大多为中低水平的遗传分化(Fst<0.15)且存在较为频繁的基因交流(Nm>>1),而太行山区的南方红豆杉种群与来自浙江、湖南和福建3省的南方红豆杉种群的分化程度较高(Fst>0.25),基因交流水平低(Nm<1)。此外,AMOVA分析显示,南方红豆杉的遗传变异主要发生在个体内,种群间和个体间的遗传变异占比较低。STRUCTURE分析和聚类分析表明,106份材料可以分成2大类,其中福建、湖南和浙江省份种源聚为一类,太行山地区的南方红豆杉聚为一类,且大多数样品间彼此相互混杂,具有较为丰富的遗传背景。【结论】南方红豆杉种群整体具有较丰富的遗传多样性,但太行山区种群的遗传多样性相对偏低。该区域内种群间呈现中低水平的遗传分化,基因交流程度较高;然而,太行山区种群与低纬度地区种群间则表现出较高的遗传分化。
【Objective】Taxus spp.are Class I protected endangered plants in China.Elucidating the genetic diversity and variation within T.chinensis var.mairei populations in the Taihang Mountain region will provide valuable insights for both conservation of genetic resources and development of improved cultivars.【Method】In this study,the genetic diversity and genetic variation of nine T.chinensis var.mairei populations in the Taihang Mountains and three populations from Zhejiang,Hunan and Fujian provinces(total of 106 individuals)were analyzed based on 8 pairs of SSR primers with good stability and polymorphism.【Result】Across the twelve T.chinensis var.mairei populations,genetic diversity analysis revealed the following averages:observed alleles(Na)=2.781,effective alleles(Ne)=2.074,Shannon information index(I)=0.679,observed heterozygosity(Ho)=0.390,and expected heterozygosity(He)=0.381.Analysis of the genetic differentiation index(Fst)and gene flow intensity(Nm)revealed predominantly low to moderate levels of genetic differentiation(Fst<0.15)and frequent gene flow(Nm>>1)among the T.chinensis var.mairei populations within the Taihang Mountains region.In contrast,higher genetic differentiation(Fst>0.25)and low gene flow(Nm<1)were observed between these Taihang Mountains populations and populations from Zhejiang,Hunan,and Fujian provinces.Furthermore,Analysis of Molecular Variance(AMOVA)revealed that the genetic variation in T.chinensis var.mairei populations occurred primarily within individuals,while relatively low proportions of variation were found among populations and among individuals.Based on STRUCTURE and cluster analyses,the 106 samples could be divided into two major groups.Accessions from Fujian,Hunan,and Zhejiang provinces clustered together,while the T.chinensis var.mairei populations from the Taihang Mountains formed a distinct cluster,and the individuals of Taihang mountain were mixed with each other and had rich genetic background.【Conclusion】This study reveals that the T.chinensis var.mairei populations collectively possess relatively high genetic diversity.However,populations within the Taihang Mountains region demonstrate lower levels of genetic diversity.Within this region,populations showed low to moderate genetic differentiation and relatively high levels of gene flow.In contrast,significant genetic differentiation was observed between the Taihang Mountains populations and those from lower-latitude regions.