文献类型：期刊文献

英文题名：In vitro Antitumor Activities of Platycarya strobilacea Sieb et Zucc Infructescence Extracts

作者：Zhang, Liangliang[1,2] Wang, Yongmei[1,2] Xu, Man[1]

第一作者：张亮亮

通信作者：Zhang, LL[1]

机构：[1]Chinese Acad Forestry, Natl Engn Lab Biomass Chem Utilizat, Inst Chem Ind Forest Prod,Key Lab Biomass Energy, Key & Open Lab Forest Chem Engn,State Forestry Ad, Nanjing 210042, Jiangsu, Peoples R China;[2]Chinese Acad Forestry, Inst New Technol Forestry, Beijing 100091, Peoples R China

年份：2014

卷号：13

期号：6

起止页码：849-854

外文期刊名：TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000341174900004)】；

基金：The authors are grateful to the Natural Science Foundation of Jiangsu, China (BK2012064) for financial support.

语种：英文

外文关键词：Platycarya strobilacea; Antitumor; Phenolic compounds; Tannins; MTT assay

摘要：Purpose: To evaluate the antitumor activities of Platycarya strobilacea infructescence extracts in A549, HepG2, SH-SY-5Y, HCT116, and U2OS-NKFB cell lines. Methods: The total amount of phenolics in P. strobilacea infructescence based on three solvent extracts (methanol, ethyl acetate and water) was measured using Folin-Ciocalteu reagent method. The cytotoxic effect of the various solvent extracts and tannins on five cancerous cell lines (A549, HepG2, SH-SY-5Y, HCT116, and U2OS-NKFB) were assayed using 3-(4,5-dimethylthiazol-2-yl)-2,5-iphenyltetrazolium bromide [MTT]. Results: Ethyl acetate extract had the highest phenolic content (725.02 mg GAE/g), followed by methanol extract (627.64 mg GAE/g). Among the extracts tested, ethyl acetate extract had effective anticancer activity against A549, HepG2, HCT116 and U2OS-NKFB cells with IC50 values of 40.1, 42.6, 40.1 and 73.4 mu g/mL, respectively. Methanol extract had the strongest cytotoxic effect on SH-SY-5Y cell with IC50 value of 31.2 mu g/mL. The number of surviving cancerous cells decreased as the concentration of the ethyl acetate and methanol extracts increased. Conclusion: The study confirms the antitumor activities of ethyl acetate and methanol extracts of P. strobilacea infructescence. Thus, the plant extracts constitute potential therapeutic materials that require further studies and development.