文献类型：期刊文献

中文题名：一种有效的蚜虫基因组DNA提取方法

英文题名：An Effective Method for Extraction Genomic DNA from Aphids

作者：杨子祥[1] 冯颖[1] 陈晓鸣[1]

第一作者：杨子祥

机构：[1]中国林业科学研究院资源昆虫研究所

年份：2005

卷号：18

期号：5

起止页码：641-642

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家科技部基础性资金项目"资源昆虫种质资源收集;整理;保存"(192)的部分内容

语种：中文

中文关键词：蚜虫;基因组DNA;提取方法

外文关键词：aphid, genomic DNA, extraction method

分类号：Q523

摘要：Aphid is a group of small insects in the Homoptera.There are approximately 4 000 aphid species all over the world.Many of them attack important agricultural crops and therefore have economic importance.However,some species such as Chinese gallnut aphids are beneficial for human beings.Most aphids display complex life cycles with alternation of sexual and asexual generations and host plant alternation.Molecular genetic markers have good stabilities,high variability and mostly are free from environmental influences.So they were very suitable for aphid research and could tackle some problems which the traditional taxonomical methods could not solve.The paper introduced a simple and effective method that could extract genomic DNA from a single aphid individual and could be used for the research of molecular genetic marker such as RAPD,RFLP and DNA sequence determination.
Aphid is a group of small insects in the Homoptera. There are approximately 4 000 aphid species all over the world. Many of them attack important agricultural crops and therefore have economic importance. However, some species such as Chinese gallnut aphids are beneficial for human beings. Most aphids display complex life cycles with alternation of sexual and asexual generations and host plant alternation. Molecular genetic markers have good stabilities, high variability and mostly are free from environmental influences. So they were very suitable for aphid research and could tackle some problems which the traditional taxonomical methods could not solve. The paper introduced a simple and effective method that could extract genomic DNA from a single aphid individual and could be used for the research of molecular genetic marker such as RAPD, RFLP and DNA sequence determination.