文献类型：期刊文献

英文题名：External browning mechanism in walnut kernel pellicles under different drying conditions based on the combination of widely-targeted and anthocyanin-targeted metabolomics

作者：Li, Qingyang[1,2] Mo, Runhong[1] Shen, Danyu[1] Sun, Shiman[1] Tang, Fubin[1] Guo, Yirong[2] Liu, Yihua[1]

第一作者：Li, Qingyang

通信作者：Liu, YH[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Peoples R China;[2]Zhejiang Univ, Inst Pesticide & Environm Toxicol, Key Lab Biol Crop Pathogens & Insects Zhejiang Pro, Key Lab Mol Biol Crop Pathogens & Insects,Minist A, Hangzhou 310058, Zhejiang, Peoples R China

年份：2024

卷号：460

外文期刊名：FOOD CHEMISTRY

收录：;EI(收录号：20243016735824);Scopus(收录号：2-s2.0-85199006488);WOS:【SCI-EXPANDED(收录号:WOS:001275935500001)】；

基金：This study was supported by the "Pioneer" and "Leading Goose" R &D Program of Zhejiang (2023C02034) and the Fundamental Research Funds of CAF (CAFYBB2019QD002) .

语种：英文

外文关键词：Walnut; Drying; Browning; Metabolome; Anthocyanin

摘要：There has been limited research on external browning (EB) of walnut. This work discovered 1888 metabolites and 34 anthocyanins in walnut pellicles (WPs) after three drying methods using widely-targeted and anthocyanin-targeted metabolomics. Based on OPLS-DA and correlation analysis, 64 temperature-responsive metabolites (TRMs; 13 anthocyanins and 51 flavonoids) were identified as critical components in relation to EB. Notably, 14 flavonoids exhibited a strong positive correlation (r > 0.9) with the browning index (BI), with upregulation of >60% after browning. Most of the identified anthocyanins were negatively linked with BI because of degradation (>45%), with correlation coefficients ranging from 0.75 to 0.97. Furthermore, anthocyanidin reductase and laccase were the two key enzymes involved in the EB of WPs, with their activities increasing by 10.57-fold and 1.32-fold, respectively, with increasing drying temperature. A metabolic pathway network of the TRM was built to provide insights into the potential mechanisms underlying EB in WPs.