文献类型：期刊文献

中文题名：没食子单宁降解菌株的筛选及发酵条件优化

英文题名：Screening of Tannic Acid Degrading Strain and Optimization of Its Fermentation Conditions

作者：闵凡芹[1,2,3,4] 王成章[1,2,3,4,5] 叶建中[1,2,3,4] 李文君[1,2,3,4] 鲁黎[6]

第一作者：闵凡芹

通信作者：Wang, Cheng-Zhang

机构：[1]中国林业科学研究院林产化学工业研究所;[2]生物质化学利用国家工程实验室;[3]国家林业局林产化学工程重点开放性实验室;[4]江苏省生物质能源与材料重点实验室;[5]中国林业科学研究院林业新技术研究所;[6]湖北省林业科学研究院

年份：2014

卷号：34

期号：5

起止页码：84-90

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;EI(收录号：20144600181403);Scopus(收录号：2-s2.0-84908572549);北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家863计划资助(2014AA021802);国际科技合作专项(2014DFR31300)

语种：中文

中文关键词：没食子单宁;优良降解菌株;筛选;发酵条件优化

外文关键词：tannic acid of Turkish galls; superior degrading strains; screening; optimization of fermentation conditions

分类号：TQ35

摘要：通过平板涂布、划线分离、菌落观察等方法,从土耳其倍子提取液自然生长的菌落中分离纯化出11株菌,从中初筛出1#、2#、4#这3株降解没食子单宁的菌株,复筛出1株没食子单宁优良降解菌株1#菌,其没食子单宁降解率达65%以上,发酵液中没食子酸质量浓度为0.052 3 g/L,单宁酶活为0.920 U/mL;对1#菌株的发酵条件进行了响应面法优化,结果表明:1#菌最佳产酶条件为培养温度31℃,初始培养基pH值5.0,最适培养时间50 h,在该条件下1#菌单宁酶酶活可达1.170 U/mL,与优化前的最大酶活0.920 U/mL相比,提高了27.2%。
3 strains of tannic acid degrading were screened out from the 11 strains which were separated from naturally fermented Turkish galls extract by the methods of spread-plate,streaking and colony observing,etc. One superior tannic acid degrading strain was found,named strain 1^#. The degradation rate of tannic acid reached beyond 65 %. In the fermentation broth,the concentration of gallic acid was 0. 052 3 g /mL and the tannase activity was 0. 920 U /mL. Fermentation conditions of strain 1^#were optimized by using Response Surface Method. The effect of temperature,initial pH,incubation time was investigated. The results showed that the optimum temperature,initial pH values and incubation time of strain 1^#was 31 ℃,5 and 50 h. Under this condition,the tannase activity was increased from 0. 920 to 1. 170 U /mL,and improved by 27. 2%.