文献类型：期刊文献

英文题名：Genome-wide analysis of the terpene synthase gene family in physic nut (Jatropha curcas L.) and functional identification of six terpene synthases

作者：Xiong, Wangdan[1,2] Wu, Pingzhi[1] Jia, Yongxia[1] Wei, Xiaoyi[1] Xu, Liangxiong[1] Yang, Yanfang[3] Qiu, Deyou[3] Chen, Yaping[1] Li, Meiru[1] Jiang, Huawu[1] Wu, Guojiang[1]

第一作者：Xiong, Wangdan

通信作者：Wu, GJ[1]

机构：[1]Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable U, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China;[2]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2016

卷号：12

期号：5

外文期刊名：TREE GENETICS & GENOMES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000385143300015)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 31270705). Thanks to Dr. Feng Chen from the University of Tennessee, Knoxville for giving some helpful suggestions and language editing. We also thank Dr. Feng Chen for kindly providing the plasmid of SmMTPSL17-TOPO.

语种：英文

外文关键词：Physic nut; Terpene synthase; Catalytic assay; Diterpene; Sesquiterpene

摘要：Plant terpenes constitute a large class of compounds and have numerous biological roles as either primary or secondary metabolites. Terpene synthases (TPSs) play key roles on catalyzing the formation of different terpenes; they are divided into seven subfamilies based on sequence relatedness (TPSa-h). TPS-a proteins catalyze the formation of sesquiterpenes and diterpenes in plants. Physic nut (Jatropha curcas L.) is an attractive biofuel tree, but its seeds contain diterpene derivatives, which make them inedible for animals. In this study, 59 putative TPS genes (JcTPS01 to JcTPS59) were identified in the physic nut genome, and 26 belong to the TPS-a subfamily. Eight among the 26 TPS-a genes showed expression in developing seeds of physic nut in the present study. After heterologous expression of these eight genes in Escherichia coli and in vitro enzyme assays, six were shown to have TPS activities. Two (JcTPS09 and JcTPS11) catalyzed the production of diterpene casbene, which was consistent with earlier findings. The other four (JcTPS02, 23, 55, and 56) catalyzed the production of sesquiterpenes. These results may facilitate the efforts for identifying TPS genes involving the physic nut terpene synthesize.