文献类型：期刊文献

中文题名：菊花DmDREBa/b基因提高转基因烟草耐逆性研究

英文题名：Study of DmDREBa/b Gene Enhanced Stress Tolerance in Transgenic Tobacco

作者：武剑[1] 杨艳芳[2] 王慧[1] 朱凯[1] 刘黎卿[3] 陈发棣[4] 喻德跃[1]

第一作者：武剑

机构：[1]南京农业大学大豆研究所国家大豆改良中心作物遗传与种质创新国家重点实验室;[2]中国林业科学研究院林业研究所林木遗传育种国家重点实验室国家林业局林木培育重点实验室;[3]福建省植物生理生化重点公共实验室福建省亚热带植物研究所;[4]南京农业大学园艺学院观赏园艺系

年份：2016

卷号：0

期号：11

起止页码：3063-3072

中文期刊名：分子植物育种

外文期刊名：Molecular Plant Breeding

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：国家自然科学基金项目(31300567;31570675);中央级公益性科研院所基本科研业务费专项(CAFYBB201-4QB001)共同资助

语种：中文

中文关键词：菊花;DREB;耐逆性;烟草转化

外文关键词：Chrysanthemum;;DREB;;Stress tolerance;;Transgenic tobacco

分类号：Q943.2

摘要：本研究组前期从菊花(Dendranthema morifolium)中克隆得到两个DREB同源基因-Dm DREBa/b,发现两者都受到低温诱导。为了进一步探究该基因的功能,本研究通过农杆菌介导的叶盘转化法将Dm DREBa/b分别导入烟草,并对这两个基因进行了耐逆性分析。结果表明,在4℃低温处理下,过表达Dm DREBa和Dm DREBb基因的烟草的鲜重和存活率都显著高于对照,并且转基因烟草的MDA含量低于对照,而POD活性高于对照;在高盐胁迫下,过表达Dm DREBb基因烟草株系的鲜重、POD活性以及脯氨酸含量都显著高于对照和过表达Dm DREBa基因烟草株系,并且过表达Dm DREBb烟草的存活率达到53%,显著高于对照的存活率20%;而对转基因烟草进行干旱胁迫后,过表达Dm DREBa烟草株系鲜重、POD活性和脯氨酸含量则均显著高于对照和过表达Dm DREBb烟草,且有60%的过表达Dm DREBa烟草恢复生长,显著高于对照(13%)和过表达Dm DREBb烟草(27%)。因此,本研究进一步证明菊花Dm DREBa和Dm DREBb基因均参与低温胁迫调控网络途径,能够提高转基因烟草耐低温能力,其中,Dm DREBa表现为更加积极调控转基因烟草的干旱胁迫,而Dm DREBb则更可能参与响应转基因烟草的高盐胁迫。
Two DREB-like genes, Dm DREBa/b had been cloned from chrysanthemum(Dendranthema morifolium)in our previous research, and both genes were induced by low temperature stress. To further study the function of these two genes, Dm DREBa/b, were overexpressed in tobacco plants by mediation of Agrobacterium tumefaciens under the control of the constitutive promoter Ca MV 35 S. The results indicated that, under low temperature treatment, the fresh weight and the survival rate of 35S: Dm DREBa and 35S: Dm DREBb tobacco plants were significantly higher than that of wild type(WT) plants. Moreover, the malondialdehyde(MDA) content of transgenic tobacco plants was lower than WT, and the peroxidase(POD) activity was higher than WT. Furthermore, the tolerance of transgenic tobacco plants was increased comparing with the WT plants at high salinity condition, and the survival rate of 35S: Dm DREBb was 53%, which was higher than that of WT(20%). The fresh weight, POD activity and the proline content of 35S: Dm DREBb were higher than that of 35S: Dm DREBa and WT, respectively. Finally,the resistant ability of transgenic plants and WT to drought stress was investigated. The results showed that the fresh weight, POD activity and the Proline content of 35S: Dm DREBa were significantly higher than that of 35S:Dm DREBb and WT, respectively, and 60% of 35S: Dm DREBa plants recoveried growth, but only 13% of WT plants and 27% of 35S: Dm DREBb plants recoveried growth. All these results indicated that Dm DREBa improved the tolerance of transgenic tobacco plants to the lower temperature and drought stresses, and Dm DREBb improved the tolerance of transgenic tobacco plants to the lower temperature and salinity stresses. This study will provide a very useful reference for functional analysis of DREB genes in chrysanthemum in the future.