文献类型：期刊文献

中文题名：离体昆虫细胞对AcNPV和BmNPV的敏感性测定与分析

英文题名：Survey and Analysis for Susceptibility of in vitro Cultured Insect Cell Lines to AcNPV and BmNPV

作者：张欣[1] 冯颖[1] 丁伟峰[1] 马涛[1]

第一作者：张欣

机构：[1]中国林业科学研究院资源昆虫研究所国家林业局资源昆虫培育与利用重点实验室

年份：2011

卷号：24

期号：3

起止页码：321-326

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：林业公益性行业科研专项(编号:4-38)

语种：中文

中文关键词：昆虫细胞系;AcNPV;BmNPV;感染

外文关键词：insect cell lines; AcNPV; BmNPV; infection

分类号：S763

摘要：采用来源于3个目14种昆虫的离体细胞系对苜蓿银纹夜蛾核型多角体病毒AcNPV(Autographa californica nuclear polyhedrosis virus)和家蚕核型多角体病毒BmNPV(Bombyx mori nuclear polyhedrosis virus)进行敏感性测定。试验结果表明:各昆虫细胞系对两种病毒的敏感性不同,来源于双翅目及直翅目的细胞系均不能被这两种病毒感染;AcNPV可侵染来源于鳞翅目的Sf21、Sf9、HighFive、RIRI-PX及HZ,宿主范围较广;而BmNPV仅能侵染BmN细胞,宿主专一。AcNPV对Sf9细胞的感染率最高,接种病毒10 d后感染率平均达84.0%,而RIRI-PX的AcNPV病毒产量最高,形成的多角体数目为43.9PIBs/感染细胞。接种BmNPV10 d后,BmN的感染率为72.2%,形成的多角体数目为23.1.PIBs/感染细胞。
Fourteen insect cell lines from three orders were used to be infected with Autographa californica nuclear polyhedrosis virus（AcNPV） and Bombyx mori nuclear polyhedrosis virus（BmNPV）.The result showed that the susceptibility for two viruses were different among different insect cell lines.The cell lines which come from Diptera and Orthoptera could not be infected with AcNPV and BmNPV.The five cell lines,Sf21,Sf9,HighFive,RIRI-PX and HZ,which come from Lepidoptera,could be infected with AcNPV.The BmN cell line could be infected only with BmNPV.The infection rate of AcNPV to Sf9 was the highest,reached 84.0% after 10 days infection.RIRI-PX infected with AcNPV could produce the highest yield of polyhedral inclusion bodies which reached 43.9 PIBs/cell at 10 day post infection.The infection rate of BmNPV to BmN was 72.2% after 10 days infection,and the yield of polyhedral inclusion bodies was 23.1PIBs/cell.