文献类型：期刊文献

中文题名：马尾松子代生长杂种优势与亲本配合力、遗传距离的相关性

英文题名：Correlation between Heterosis in the Growth of Progeny and Combining Ability and Genetic Distance of the Parents for Pinus massoniana

作者：董虹妤[1] 刘青华[1] 周志春[1] 金国庆[1] 沈丹玉[1] 宋新回[2]

第一作者：董虹妤

通信作者：Liu, Qinghua

机构：[1]中国林业科学研究院亚热带林业研究所国家林业局马尾松工程技术研究中心浙江省林木育种技术研究重点实验室;[2]浙江省淳安县姥山林场

年份：2017

卷号：53

期号：2

起止页码：65-75

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;EI(收录号：20172303730451);Scopus(收录号：2-s2.0-85020087186);北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金："十二五"国家科技支撑计划课题(2012BAD01B02);国家自然科学基金项目(31100491);浙江省农业新品种选育重大科技专项项目重点课题(2012C12908-12);国家林业公益性行业专项(201204307)

语种：中文

中文关键词：马尾松;种质;杂种优势;GCA;SCA;遗传距离

外文关键词：Pinus massoniana; germplasm; heterosis; GCA; SCA; genetic distance

分类号：S718.46

摘要：【目的】开展马尾松子代生长杂种优势与亲本配合力、遗传距离的相关性研究,以根据子代的预测结果对马尾松杂交育种亲本进行科学选配。【方法】利用设置在浙江省淳安县姥山林场的2份马尾松第3代6×6半双列杂交材料,分析子代生长性状的杂种优势和亲本配合力,并利用SSR标记分析杂交亲本间的遗传距离,探究子代生长性状杂种优势与亲本一般配合力(GCA)、特殊配合力(SCA)、遗传距离(GD)的相关关系。【结果】2份马尾松试验材料树高、胸径和材积指数的表型值与杂种优势在不同杂交组合间差异较大,除半双列Ⅱ胸径一般配合力(GCA)外,其余生长性状配合力在杂交组合间的差异均达到显著或极显著水平。除半双列Ⅱ的胸径外,其余马尾松生长性状均是以加性基因效应控制为主。各生长性状家系遗传力(h_f^2)水平较高,为0.884~0.966,受较强的遗传控制。2份试验材料中12个亲本的Nei’s基因多样度指数和Shannon信息多样性指数分别为0.301 2和0.467 8,遗传多样性处于中等水平,不同亲本间GD为0.142 1~0.351 6。相关性分析发现,多数马尾松子代生长性状表型值及其杂种优势与GCA、SCA、GD(≤0.351 6)呈较显著的正相关关系。亲本GCA中,亲本一般配合力之和(GCA_(P1+P2))对子代表型值和杂种优势的预测能力最强,父本一般配合力(GCA_(P2))次之,而母本一般配合力(GCAP1)预测能力最差。亲本GCA_(P1+P2)、SCA、GD中,GCA_(P1+P2)对马尾松子代生长性状表型值的预测能力最强,SCA对马尾松子代生长性状杂种优势的预测能力最强,而GD对子代生长性状表型值和杂种优势的预测能力普遍弱于GCA_(P1+P2)和SCA。回归分析发现,2份试验材料中,除半双列Ⅱ的GCA_(P1+P2)外,其余GCA_(P1+P2)、SCA、GD(≤0.351 6)与子代材积指数的杂种优势均呈较显著的线性相关。【结论】GCA_(P1+P2)、SCA以及较低水平的GD与马尾松子代生长性状表型值和杂种优势的相关性较高,可以利用亲本配合力和一定水平的遗传距离对马尾松子代生长性状表型值和杂种优势进行有效预测,进而提高马尾松亲本选配效率及杂种优势的有效利用率。
【Objective】The correlations between heterosis in growth traits of progeny and combining ability,genetic distance of their parents for Pinus massoniana were analyzed to select parents scientifically for hybrid breeding based on performance of the progeny. The study on heterosis,combining ability and genetic distance would provide guidance for the breeding of Pinus massoniana. 【Method】Two sets of test materials from the third generation of P. massoniana grown in Laoshan Forest Farm of Zhejiang Province were used to investigate the heterosis and combining ability of growth traits in6 × 6 half diallel cross designs. SSR markers were used to analyze the genetic distances between mating parents and the relationships between heterosis of the progeny and general combining ability（GCA）,specific combining ability（SCA）,genetic distance（GD） of the mating parents. 【Result】Results showed that the phenotypic value and heterosis of growthtraits exhibited significant differences between the two hybrid combinations. Except the GCA of DBH in the design II,the combining abilities of other growth traits were significantly different between different mating combinations. All growth traits but DBH in the design II were controlled mainly by the additive gene effects. For all growth traits,family heritability（hf^2） were relatively high（0. 884-0. 966）,indicating a high level of genetic control for the growth traits. Genetic diversity of the 12 breeding parents was at a moderate level with Nei＇s gene diversity index of 0. 301 2 and Shannon＇s information diversity index of 0. 467 8. The GDs among parents were 0. 142 1-0. 351 6. Meanwhile,most correlations between phenotypic value,heterosis of growth traits and the sum of general combining ability（GCA（P1＋P2））,SCA,GD（≤0. 351 6） were significantly positive. Among the GCAs of mating parents,GCA（P1＋P2） was proved to have the strongest predictive ability for the phenotypic value and heterosis of growth traits,the general combining ability of male parents（GCA（P2）） was the second,and the general combining ability of female parents（GCAP1） was the weakest. Among the GCA（P1＋P2）,SCA and GD of the parents,GCA（P1＋P2） was proved to have the strongest ability to predict the phenotypic value of growth traits,SCA was proved to have the strongest ability to predict the heterosis,and the predictive ability of GD for phenotypic value and heterosis was weaker than GCA（P1＋P2） and SCA. Except the GCA（P1＋P2） of cross designⅡ,other GCA（P1＋P2）,SCA,GD were found to have a significant linear correlation with the heterosis in volume index of the progeny. 【Conclusion】Since there existed a relative closer correlation between GCA（P1＋P2）,SCA,GD（≤0. 351 6） and phenotypic value,heterosis of growth traits,it is feasible to predict the phenotypic value and heterosis in growth traits by combining ability and smaller genetic distance to improve the efficiency of parental apolegamy and the utilization of heterosis for P. massoniana.