文献类型：期刊文献

中文题名：靛蓝提取物中靛蓝和靛玉红的分析方法比较

英文题名：Comparation of Three Methods of Analytical Methodology of Indigo and Indirubin in Different Indigo Extracts

作者：王成章[1] 颜洋洋[1,2] 周昊[1] 刘丹阳[1] 商士斌[1] 张华兴[3]

第一作者：王成章

机构：[1]中国林业科学研究院林产化学工业研究所,江苏省生物质能源与材料重点实验室,国家林业和草原局林产化学工程重点实验室,林木生物质低碳高效利用国家工程研究中心,江苏南京210042;[2]南京林业大学江苏省林业资源高效加工利用协同创新中心,江苏南京210037;[3]凯里小生物科技有限公司,贵州凯里556000

年份：2024

卷号：58

期号：1

起止页码：18-26

中文期刊名：生物质化学工程

外文期刊名：Biomass Chemical Engineering

收录：CSTPCD

基金：中国林科院中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2021ZC003);贵州科技支撑项目(黔科合支撑[2022]-159)。

语种：中文

中文关键词：靛蓝;靛玉红;统计分析;HPLC法;UV光谱法

外文关键词：indigo;indirubin;statistic analysis;HPLC method;UV spectrometry

分类号：TQ35;TQ91

摘要：以靛膏为原料,采用盐酸酸化、葡萄糖还原和乙酸乙酯提取3种工艺制备靛蓝酸化物、靛蓝还原物和乙酸乙酯提取物。建立高效液相色谱(HPLC)法、紫外可见分光光度(UV)法和双波长紫外分光光度(dW-UV)法,分析3种靛蓝提取物中靛蓝和靛玉红的含量,并以Kolmogorov-Smirnov检验、Bland-Altman偏差图比较和Spearman检验确定3种样品各自最佳分析方法。结果表明:所建立的HPLC法、UV法和dW-UV法简单、准确、灵敏度高、稳定性好和重复性好。3种靛蓝提取物成分含量检测方法均遵从正态分布,两两测定值存在显著差异。UV法更适于分析靛蓝酸化物、靛蓝还原物中靛蓝含量;HPLC法更适于乙酸乙酯提取物中靛蓝、靛玉红含量分析。
Using indigo paste as raw material,indigo acidification substances,glucose reducing substances,and ethyl acetate extractives were prepared using three processes of hydrochloric acid acidification,glucose reduction,and ethyl acetate extraction,respectively.High performance liquid chromatography(HPLC),ultraviolet visible spectrophotometry(UV),and dual wavelength ultraviolet spectrophotometry(dW-UV)were established to analyze the contents of indigo and indirubin in samples from three processes.The optimal analysis methods for each of three samples were determined through Kolmogorov Smirnov test,comparison of Bland Altman deviation plots,and Spearman test.The results showed that the established HPLC,UV,and dWUV methods were simple,accurate,highly sensitive,stable,and reproducible.Three methods for detecting the content of indigo extract components all followed the normal distribution,and there were significant differences between each the two measured values.The UV method was more suitable for analyzing the content of indigo in indigo acidified substances and indigo reducing substances.HPLC method was more suitable for measuring the content of indigo and indirubin in ethyl acetate extract.