文献类型：期刊文献

中文题名：HPLC-MS/MS法同时测定马尾松松针中水杨酸和茉莉酸

英文题名：Simultaneous determination of salicylic acid and jasmonic acid in pinus massoninan by high performance liquid chromatography-tandem mass spectrometry

作者：钟冬莲[1,2] 任传义[1] 李祖光[2] 刘青华[1] 莫润宏[1] 沈丹玉[1] 汤富彬[1]

第一作者：钟冬莲

机构：[1]中国林科院亚热带林业研究所;[2]浙江工业大学化学工程学院

年份：2017

卷号：36

期号：2

起止页码：164-167

中文期刊名：分析试验室

外文期刊名：Chinese Journal of Analysis Laboratory

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

语种：中文

中文关键词：水杨酸;茉莉酸;HPLC-MS/MS;固相萃取;马尾松

外文关键词：Salicylic acid; Jasmonic acid; High performance liquid chromatography-tandem mass spectrometry; Solid phase extraction; Pinus massoninan

分类号：S132

摘要：建立了SPE-HPLC-MS/MS同时测定新鲜马尾松松针中水杨酸和茉莉酸2种内源植物激素的方法。新鲜马尾松松针样品经液氮磨碎后,用甲醇/水/甲酸(80:19:1,V/V/V)溶液涡旋提取,经混合型阳离子交换小柱(Oasis MCX)净化,采用Symmetry C18(3.9 mm×150 mm,5μm)色谱柱分离,以水(含0.1%甲酸(V/V)和10 mmol/L甲酸铵)和甲醇为流动相,梯度洗脱,采用电喷雾电离,负离子扫描多反应监测模式(MRM)进行检测,2种植物激素均采用相应的同位素内标进行定量。结果表明,水杨酸和茉莉酸2种目标化合物的方法检出限分别为1.5μg/kg和1.0μg/kg,线性范围为2~100μg/L(r>0.995),添加回收率为78.5%~96.2%,RSD为5.7%~11%。方法实现了同时测定新鲜马尾松松针中水杨酸和茉莉酸,可推广应用于其它植物组织中水杨酸和茉莉酸含量的分析。
This paper describes a method to detect and quantitate the endogenous plant hormones salicylic acid and jasmonic acid by means of solid phase extraction and high performance liquid chromatography-tandem mass spectrometry in fresh leaves of pinus massoninan.Deuterium-labeled[2H5]（±）-jasmonic acid and [2H4]-salicylic acid were used as internal standards.Fresh leaves of Pinus massoninan（1 g） were cryogenically grinded by liquid nitrogen,extracted with methanol/water/formic acid（80：19：1）,and then purified on an Oasis MCX SPE cartridge.The separation was performed on a Symmetry C18 column by using methanol and water（containing 0.1% formic acid and10 mmol/L ammonium formate） as mobile phases with gradient elution.The analytes were detected by tandem mass spectrometry in multiple reaction monitoring（MRM） mode.The electrospray ionization（ESI） source was used for the quantitative analysis in negative mode.The results showed that the limits of detection（LODs） for salicylic acid and jasmonic acid were 1.5 μg/kg and 1.0 μg/kg.The calibration curves of the two analytes were linear in the range of 2 - 100 μg/L with the correlation coefficients higher than 0.995.The respective mean recoveries were found to be in the range of 78.5%- 96.2%,and the RSDs were 5.7% - 11%.It was applicable to the analysis of salicylic acid and jasmonic acid in other plant issues.This method is simple,fast,sensitive and accurate.