文献类型：期刊文献

英文题名：In Vivo and In Vitro Toxicity Evaluation of Polyprenols Extracted from Ginkgo biloba L. Leaves

作者：Wang, Cheng-Zhang[1,2,3,4] Yuan, Jiao-Jiao[1,2,3,4] Li, Wen-Jun[1,2,3,4] Zhang, Hong-Yu[1,2,3,4] Ye, Jian-Zhong[1,2,3,4]

第一作者：Wang, Cheng-Zhang;王成章

通信作者：Wang, CZ[1]

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Nanjing 210042, Jiangsu, Peoples R China;[2]State Forestry Adm, Key & Open Lab Forest Chem Engn, Nanjing 210042, Jiangsu, Peoples R China;[3]Inst Chem Ind Forest Prod, Key Lab Biomass Energy & Mat, Nanjing 210042, Jiangsu, Peoples R China;[4]Chinese Acad Forestry, Inst New Technol Forestry, Beijing 100091, Peoples R China

年份：2015

卷号：20

期号：12

起止页码：22257-22271

外文期刊名：MOLECULES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000367533400094)】；

基金：The study was conducted at Jiangsu provincial center for disease control and prevention. Financial support by the international cooperation program "No.2014DFR31300" was thankfully acknowledged for this work.

语种：英文

外文关键词：polyprenols; Ginkgo biloba leaves; toxicity; acute toxicity; subchronic toxicity

摘要：Polyprenols of Ginkgo biloba L. leaves (GBP) are a new type of lipid with 14-24 isoprenyl units, which in humans have strong bioactivity like the dolichols. A large amount of work showed that GBP had good antibacterial activity and powerful protective effects against acute hepatic injury induced by carbon tetrachloride and alcohol, as well as antitumor activity, but the safety of GBP was not considered. The current study was designed to evaluate the toxicity of these polyprenols. Acute toxicity in mice was observed for 14 days after GBP oral dosing with 5, 7.5, 10, 15 and 21.5 g/kg body weight (b. wt.) Further, an Ames toxicity assessment was carried out by plate incorporation assay on spontaneous revertant colonies of TA97, TA98, TA100 and TA102, with GBP doses designed as 8, 40, 200, 1000 and 5000 g/dish, and subchronic toxicity was evaluated in rats for 91 days at GBP doses of 500, 1000 and 2000 mg/kg b. wt./day. The weight, food intake, hematological and biochemical indexes, the ratio of viscera/body weight, and histopathological examinations of tissue slices of organs were all investigated. The results showed that no animal behavior and appearance changes and mortality were seen during the observation period with 21.5 g/kg GBP dose in the acute toxicity test. Also, no mutagenicity effects were produced by GBP (mutation rate < 2) on the four standard Salmonella strains (p > 0.05) in the Ames toxicity test. Furthermore, the no observed adverse effect level (NOAEL) of GBP was 2000 mg/kg for 91 days feeding of rats in the subchronic toxicity tests. Results also showed the hematological and biochemical indexes as well as histopathological examination changed within a small range, and all clinical observation indexes were normal. No other distinct impacts on cumulative growth of body weight, food intake and food utilization rate were discovered with GBP. No significant difference was discovered for the rats' organ weight and the ratio of viscera to body weight (p > 0.05). Reversible pathological changes in the histopathological examinations of tissue slices of organs were not observed. GBP could therefore be considered as a safe material with minor side effects.