文献类型：期刊文献

英文题名：Insight into growth and wood properties based on QTL and eQTL mapping in Populus deltoides 'Danhong' x Populus simonii 'Tongliao1'

作者：Zhang, Lei[1,2] Lu, Dongye[3] Ge, Xiaolan[1] Du, Jiujun[1] Wen, Shuangshuang[1] Xiang, Xiaodong[1] Du, Changjian[1] Zhou, Xinglu[1] Hu, Jianjun[1,2]

第一作者：张雷;Zhang, Lei

通信作者：Hu, JJ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,Natl Forestry Gra, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Jiangsu, Peoples R China;[3]Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing 100093, Peoples R China

年份：2023

卷号：199

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000983690800001)】；

基金：Acknowledgments This work was supported by National Key Research and Develop-ment Program of China (2021YFD2200201) , and National Natural Sci-ence Foundation of China (32071797) .

语种：英文

外文关键词：Populus; QTL; eQTL; Xylem development; Co-expression network; Calmodulin

摘要：Poplar is a short-rotation cultivated energy tree species, and analysis of genetic regulation of gene expression at the seedling stage is important for understanding phenotypic variation in growth rate and wood quality. How-ever, the genetic regulation of xylem gene expression is still poorly understood. We determined transcriptomes of developing xylem of Populus deltoides 'Danhong', P. simonii 'Tongliao1' and F1 hybrids of them to explore the variations in the landscape transcriptome and its genetic basis. Through quantitative trait locus (QTL) and expression QTL (eQTL) analysis, we identified 237 QTLs, 48,019 cis-eQTLs and 2,184,889 trans-eQTLs contributing to the whole-genome transcriptome variation in poplar. Trans-acting effects play major role in expression regulation. We identified 38 eQTL hotspots, among which the A/T base mutation in PdCaM247 3 ' UTR in hot12654 influenced the wood basic density and regulated the expression of QTL major genes (YEATS and IQD32) and secondary cell wall related genes. Transgenic poplar with overexpressing PdCaM247 presented an increase of the secondary xylem. Yeast two-hybrid analysis revealed that PdCaM247 interacted with the sec-ondary cell wall regulator MYB156. This study generates a large genetic resource for studying xylem develop-ment and provides new insights into the genetic basis of secondary cell wall regulatory networks.