文献类型：期刊文献

英文题名：PdRabG3f interfered with gibberellin-mediated internode elongation and xylem developing in poplar

作者：Du, Jiujun[1] Wei, Hantian[1] Song, Xueqin[1,2] Zhang, Lei[1,2] Hu, Jianjun[1,2]

第一作者：Du, Jiujun

通信作者：Zhang, L[1];Hu, JJ[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, CoInnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Jiangsu, Peoples R China

年份：2024

卷号：343

外文期刊名：PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85189006914);WOS:【SCI-EXPANDED(收录号:WOS:001289811700001)】；

基金：This research was supported by the Major Project of Agricultural Biological Breeding (2023ZD04072), the National Key Research and Development Program of China (2021YFD2200201), and the National Natural Science Foundation of China (32071797).

语种：英文

外文关键词：Populus; QTL; Internode elongation; Xylem; Cell wall; Gibberellin

摘要：As a member of the small GTPases family, Rab GTPases play a key role in specifying transport pathways in the intracellular membrane trafficking system and are involved in plant growth and development. By quantitative trait locus (QTL) mapping, PdRabG3f was identified as a candidate gene associated with shoot height in a hybrid offspring of Populus deltoides 'Danhong' x Populus simonii 'Tongliao1'. PdRabG3f localized to the nucleus, endoplasmic reticulum and tonoplast and was primarily expressed in the xylem and cambium. Overexpression of PdRabG3f in Populus alba x Populus glandulosa (84 K poplar) had inhibitory effects on vertical and radical growth. In the transgenic lines, there were evident changes in the levels of 15 gibberellin (GA) derivatives, and the application of exogenous GA3 3 partially restored the phenotypes mediated by GAs deficiency. The interaction between PdRabG3f and RIC4, which was the GA-responsive factor, provided additional explanation for PdRabG3f's inhibitory effect on poplar growth. RNA-seq analysis revealed differentially expressed genes (DEGs) associated with cell wall, xylem, and gibberellin response. PdRabG3f interfering endogenous GAs levels in poplar might involve the participation of MYBs and ultimately affected internode elongation and xylem development. This study provides a potential mechanism for gibberellin-mediated regulation of plant growth through Rab GTPases.