文献类型：期刊文献

英文题名：PeCLH2 Gene Positively Regulate Salt Tolerance in Transgenic Populus alba x Populus glandulosa

作者：Ge, Xiaolan[1,2] Du, Jiujun[1] Zhang, Lei[1,3] Qu, Guanzheng[2] Hu, Jianjun[1,3]

第一作者：Ge, Xiaolan

通信作者：Hu, JJ[1];Hu, JJ[2]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Xiangshan Rd, Beijing 100091, Peoples R China;[2]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Hexing Rd, Harbin 150040, Peoples R China;[3]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry Southe, Nanjing 210037, Peoples R China

年份：2023

卷号：14

期号：3

外文期刊名：GENES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000956252200001)】；

语种：英文

外文关键词：Populus euphratica; PeCLH2; salt stress; physiologic indexes; transcriptome

摘要：Salt is an important environmental stress factor, which seriously affects the growth, development and distribution of plants. Chlorophyllase plays an important role in stress response. Nevertheless, little is known about the physiological and molecular mechanism of chlorophyll (Chlase, CLH) genes in plants. We cloned PeCLH2 from Populus euphratica and found that PeCLH2 was differentially expressed in different tissues, especially in the leaves of P. euphratica. To further study the role of PeCLH2 in salt tolerance, PeCLH2 overexpression and RNA interference transgenic lines were established in Populus alba x Populus glandulosa and used for salt stress treatment and physiologic indexes studies. Overexpressing lines significantly improved tolerance to salt treatment and reduced reactive oxygen species production. RNA interference lines showed the opposite. Transcriptome analysis was performed on leaves of control and transgenic lines under normal growth conditions and salt stress to predict genes regulated during salt stress. This provides a basis for elucidating the molecular regulation mechanism of PeCLH2 in response to salt stress and improving the tolerance of poplar under salt stress.