文献类型：期刊文献

英文题名：Genome-Wide Analysis of the AAAP Gene Family in Populus and Functional Analysis of PsAAAP21 in Root Growth and Amino Acid Transport

作者：Du, Jiujun[1] Du, Changjian[1] Ge, Xiaolan[1] Wen, Shuangshuang[1] Zhou, Xinglu[1] Zhang, Lei[1,2] Hu, Jianjun[1,2]

第一作者：Du, Jiujun

通信作者：Zhang, L[1];Hu, JJ[1];Zhang, L[2];Hu, JJ[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Res Inst Forestry,Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry Southe, Nanjing 210037, Peoples R China

年份：2023

卷号：24

期号：1

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000909283100001)】；

基金：This study was supported by the National Key Research and Development Program of China (2021YFD2200201) and the National Natural Science Foundation (32071797).

语种：英文

外文关键词：Populus; PtrAAAPs; adventitious root; amino acid transport; QTL

摘要：The adventitious root (AR) is the basis for successful propagation by plant cuttings and tissue culture and is essential for maintaining the positive traits of a variety. Members of the amino acid/auxin permease (AAAP) gene family play indispensable roles in various plant metabolisms and have few studies on root growth and amino acid transport. In this study, with a systematic bioinformatics analysis of the Populus AAAP family, 83 PtrAAAPs were identified from Populus trichocarpa and grouped into 8 subfamilies. Subsequently, chromosomal distribution, genetic structure, cis-elements analysis, and expression pattern analysis of the AAAP family were performed and the potential gene AAAP21 regulating root development was screened by combining the results of RNA-Seq and QTL mapping. PsAAAP21 was proven as promoting root development by enhancing AR formation. Differentially expressed genes (DEGs) from RNA-seq results of overexpressing lines were enriched to multiple amino acid-related pathways, and the amino acid treatment to transgenic lines indicated that PsAAAP21 regulated amino acid transport, including tyrosine, methionine, and arginine. Analysis of the AAAP gene family provided a theoretical basis for uncovering the functions of AAAP genes. The identification of PsAAAP21 on root promotion and amino acid transport in Populus will help with breeding new woody plant species with strong rooting ability.