文献类型：期刊文献

英文题名：Insight into growth and wood properties based on QTL and eQTL mapping in Populus deltoides ‘Danhong’ × Populus simonii ‘Tongliao1’

作者：Zhang, Lei[1,2] Lu, Dongye[3] Ge, Xiaolan[1] Du, Jiujun[1] Wen, Shuangshuang[1] Xiang, Xiaodong[1] Du, Changjian[1] Zhou, Xinglu[1] Hu, Jianjun[1,2]

第一作者：Zhang, Lei;张雷

机构：[1] State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of National Forestry and Grassland Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China; [2] Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Jiangsu, Nanjing, 210037, China; [3] Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100093, China

年份：2023

卷号：199

外文期刊名：Industrial Crops and Products

收录：EI(收录号：20231713947721);Scopus(收录号：2-s2.0-85152947478)

语种：英文

外文关键词：Quality control - Wood

摘要：Poplar is a short-rotation cultivated energy tree species, and analysis of genetic regulation of gene expression at the seedling stage is important for understanding phenotypic variation in growth rate and wood quality. However, the genetic regulation of xylem gene expression is still poorly understood. We determined transcriptomes of developing xylem of Populus deltoides ‘Danhong’, P. simonii ‘Tongliao1’ and F1 hybrids of them to explore the variations in the landscape transcriptome and its genetic basis. Through quantitative trait locus (QTL) and expression QTL (eQTL) analysis, we identified 237 QTLs, 48,019 cis-eQTLs and 2,184,889 trans-eQTLs contributing to the whole-genome transcriptome variation in poplar. Trans-acting effects play major role in expression regulation. We identified 38 eQTL hotspots, among which the A/T base mutation in PdCaM247 3′UTR in hot12654 influenced the wood basic density and regulated the expression of QTL major genes (YEATS and IQD32) and secondary cell wall related genes. Transgenic poplar with overexpressing PdCaM247 presented an increase of the secondary xylem. Yeast two-hybrid analysis revealed that PdCaM247 interacted with the secondary cell wall regulator MYB156. This study generates a large genetic resource for studying xylem development and provides new insights into the genetic basis of secondary cell wall regulatory networks. ? 2023 Elsevier B.V.