文献类型：期刊文献

英文题名：An efficient approach to finding Siraitia grosvenorii triterpene biosynthetic genes by RNA-seq and digital gene expression analysis

作者：Tang, Qi[1,3] Ma, Xiaojun[1,3] Mo, Changming[3] Wilson, Iain W.[4] Song, Cai[3] Zhao, Huan[1] Yang, Yanfang[2] Fu, Wei[1] Qiu, Deyou[2]

第一作者：Tang, Qi

通信作者：Ma, XJ[1]

机构：[1]Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant, Beijing 100193, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[3]Chinese Acad Med Sci, Inst Med Plant Dev, Guangxi Branch Inst, Nanning 530023, Peoples R China;[4]CSIRO Plant Ind, Canberra, ACT 2001, Australia

年份：2011

卷号：12

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000294292000002)】；

基金：We acknowledge the Beijing Genomics Institute at Shenzhen for its assistance in original data processing and related bioinformatics analysis. This work was supported by the National Natural Science Foundation of China (grant No. 30860379 & 30960500).

语种：英文

摘要：Background: Siraitia grosvenorii (Luohanguo) is an herbaceous perennial plant native to southern China and most prevalent in Guilin city. Its fruit contains a sweet, fleshy, edible pulp that is widely used in traditional Chinese medicine. The major bioactive constituents in the fruit extract are the cucurbitane-type triterpene saponins known as mogrosides. Among them, mogroside V is nearly 300 times sweeter than sucrose. However, little is known about mogrosides biosynthesis in S. grosvenorii, especially the late steps of the pathway. Results: In this study, a cDNA library generated from of equal amount of RNA taken from S. grosvenorii fruit at 50 days after flowering (DAF) and 70 DAF were sequenced using Illumina/Solexa platform. More than 48,755,516 high-quality reads from a cDNA library were generated that was assembled into 43,891 unigenes. De novo assembly and gap-filling generated 43,891 unigenes with an average sequence length of 668 base pairs. A total of 26,308 (59.9%) unique sequences were annotated and 11,476 of the unique sequences were assigned to specific metabolic pathways by the Kyoto Encyclopedia of Genes and Genomes. cDNA sequences for all of the known enzymes involved in mogrosides backbone synthesis were identified from our library. Additionally, a total of eighty-five cytochrome P450 (CYP450) and ninety UDP-glucosyltransferase (UDPG) unigenes were identified, some of which appear to encode enzymes responsible for the conversion of the mogroside backbone into the various mogrosides. Digital gene expression profile (DGE) analysis using Solexa sequencing was performed on three important stages of fruit development, and based on their expression pattern, seven CYP450s and five UDPGs were selected as the candidates most likely to be involved in mogrosides biosynthesis. Conclusion: A combination of RNA-seq and DGE analysis based on the next generation sequencing technology was shown to be a powerful method for identifying candidate genes encoding enzymes responsible for the biosynthesis of novel secondary metabolites in a non-model plant. Seven CYP450s and five UDPGs were selected as potential candidates involved in mogrosides biosynthesis. The transcriptome data from this study provides an important resource for understanding the formation of major bioactive constituents in the fruit extract from S. grosvenorii.