文献类型：期刊文献

英文题名：Molecular analysis of proanthocyanidins related to pigmentation in brown cotton fibre (Gossypium hirsutum L.)

作者：Feng, Hongjie[1] Li, Yanjun[1] Wang, Shaofang[2] Zhang, Liangliang[3] Liu, Yongchuang[1] Xue, Fei[1] Sun, Yuqiang[4] Wang, Yongmei[3] Sun, Jie[1]

第一作者：Feng, Hongjie

通信作者：Sun, J[1]

机构：[1]Shihezi Univ, Coll Agr, Key Lab Oasis Ecoagr, Shihezi 832000, Xinjiang Provin, Peoples R China;[2]Univ Western Australia, Ctr Legumes Mediterranean Agr, Crawley, WA 6009, Australia;[3]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Nanjing 210042, Jiangsu, Peoples R China;[4]Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310036, Zhejiang, Peoples R China

年份：2014

卷号：65

期号：20

起止页码：5759-5769

外文期刊名：JOURNAL OF EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000344658800005)】；

基金：We thank Professors Guixian Xia and Haiyun Wang (Institute of Microbiology, Chinese Academy of Sciences, China) for their kind assistance with experiments and critical reading of the manuscript. We also thank Dr William J. Gale (College of Agriculture, Shihezi University, China) and Dr David Harris (Australia) for critical reading and professional editing of the manuscript. This work was supported by the National Basic Research Priorities Program (grant no: U1303281) and the National Key Basic Research and Development Program (973 Program) (no. 2007CB116210).

语种：英文

外文关键词：Anthocyanidin reductase (ANR); brown cotton; nuclear magnetic resonance (NMR); pigment; proanthocyanidin; quinone

摘要：The structural characteristics and component differences of proanthocyanidins in brown and white cotton fibres were identified by nuclear magnetic resonance (NMR) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) analyses. Proanthocyanidins in brown and white cotton fibres were found to contain mainly procyanidin (PC) and prodelphidin (PD) units with 2, 3-cis form (epigallocatechin and epicatechin). However, part of the proanthocyanidins in the white cotton fibres were modified by acylation and were constitutively different from the proanthocyanidins in brown cotton fibres. The relative amount of PD was similar to that of PC in white cotton fibres, while proanthocyanidins in brown cotton fibres consisted mainly of PD units with a relative ratio of 9:1. In brown cotton fibres, the proanthocyanidin monomeric composition was consistent with the expression profiles of proanthocyanidin synthase genes, suggesting that anthocyanidin reductase represented the major flow of the proanthocyanidin biosynthesis pathway. In addition, the structural characteristics and component differences of proanthocanidins in brown and white cotton fibres suggested that quinones, the oxidation products of proanthocyanidins, were the direct contributors to colour development in brown cotton fibre. This was demonstrated by vanillin-HCl staining and Borntrager's test. Collectively, these data demonstrated that the biosynthesis of proanthocyanidins is a crucial pigmentation process in brown cotton fibre, and that quinones may represent the main pigments contributing to formation of the the brown colour. This study revealed the molecular basis of pigmentation in brown cotton fibres, and provided important insights for genetic manipulation of pigment production in cotton fibres.