文献类型：期刊文献

中文题名：白蜡虫多糖的提取及单糖组分分析

英文题名：The Extraction of Polysaccharide from White Wax Scale and Analysis of Monosaccharide Compositions

作者：何钊[1] 孙龙[1] 冯颖[1] 陈晓鸣[1]

第一作者：何钊

机构：[1]中国林业科学研究院资源昆虫研究所国家林业局资源昆虫培育与利用实验室

年份：2008

卷号：21

期号：6

起止页码：792-796

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金："十一五"国家科技支撑计划重点项目(2006BAD06B07);国家林业局引进国外先进农业技术项目(20064116)部分研究内容

语种：中文

中文关键词：白蜡虫;多糖;毛细管电泳;单糖;衍生

外文关键词：white wax scale, polysaccharide, CZE, monosaccharide, derivation

分类号：S899.1

摘要：采用水提醇沉法对资源昆虫——白蜡虫中水溶性多糖组分进行提取,测定了提取物的多糖含量、蛋白含量和氨基酸含量;以1-苯基-3-甲基-5-吡唑啉酮(PMP)为柱前衍生化试剂,利用毛细管区带电泳(CZE)对其多糖的单糖组成及摩尔比率进行分析,对电泳缓冲液的浓度和pH值进行了优化。结果表明:白蜡虫水溶性多糖的提取率为2.1%,多糖含量为256 mg.g-1,蛋白含量为242 mg.g-1;白蜡虫多糖由葡萄糖、甘露糖及半乳糖3种单糖组成,其摩尔比为1∶0.85∶0.93。
White Wax Scale （Ericerus pela Chavannes） polysaccharide was extracted with water, and precipitated by alcohol. The contents of polysaecharide,protein and amino acids were measured. The capillary zone electrophoresis （CZE） method was developed to separate and identify the monosaccharide composition of white wax scale polysaccharide. In this method, white wax scale polysaccharide was hydrolyzed into component monosaecharides and subsequently derived with 1-phenyl-3-methyl-5-pyrazolone （ PMP）, the concentration and pH of buffer were studied and optimized. The results showed extracting ratio of polysaceharide was 2.4%, content of polysaccharide was 256 mg · g^- 1 content of protein was 242 mg · g^- 1, the polysaccharide consisted of glucose, mannose, galactose with the molar ratio of 1 ： 0.85： 0.93.