文献类型：期刊文献

中文题名：壶瓶枣多糖的分离及其抗氧化活性

英文题名：Purification of polysaccharides from Ziziphus Jujuba Mill. cv. Hupingzao and the antioxidant activities

作者：张耀雷[1] 黄立新[1] 张彩虹[1,2] 谢普军[1] 游凤[1]

第一作者：张耀雷

机构：[1]中国林业科学研究院林产化学工业研究所,生物质化学利用国家工程实验室,国家林业局林产化学工程重点开放性实验室,江苏省生物质能源与材料重点实验室,江苏南京210042;[2]中国林业科学院林业新技术研究所,北京100091

年份：2015

卷号：37

期号：6

起止页码：1267-1271

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：国家林业局948项目(2012-4-12);中央级公益性科研院所基本科研业务费专项资金(CAFINT2013C04)

语种：中文

中文关键词：壶瓶枣;多糖;纯化;抗氧化活性

外文关键词：Ziziphus Jujuba Mill. cv. Hupingzao ; polysaccharide ; purification ; antioxidant activity

分类号：R284.1

摘要：目的研究壶瓶枣多糖的分离纯化及其抗氧化活性。方法壶瓶枣粗多糖用D900型大孔吸附树脂和DEAE-纤维素-52柱层析分离,以去离子水和0~1 mol/L的Na Cl溶液梯度洗脱,并考察其不同组分清除DPPH自由基、羟基自由基和超氧阴离子自由基的能力。结果分离纯化得到3个中性多糖组分（ZJP-1、ZJP-3和ZJP-5）和2个酸性多糖组分（ZJP-2和ZJP-4）,并对DPPH自由基均有一定的清除能力。其中ZJP-2清除超氧阴离子自由基能力较强;ZJP-4清除羟基自由基能力较强;ZJP-5清除以上两种自由基能力均较强。结论 D900型大孔吸附树脂和DEAE-纤维素-52的分离纯化效果较好,而且分离所得的5种多糖组分的抗氧化活性各有特点。
AIM To study the isolation and purification of polysaccharides from Ziziphus Jujuba Mill. cv. Hupingzao and the antioxidant activity of these polysaccharides. METHODS The crude polysaccharides were pu- rified by macroporous adsorption resin D900 and DEAE-cellulose-52 column chromatography, eluted with deionized water and 0 - 1 mol/L NaC1 solution. The scavenging capacities of DPPH, hydroxy- and superoxide- free radicals were also measured. RESULTS Three neutral polysaccharide fractions （ZJP-1, ZJP-3, ZJP-5 ） and two acidic polysaccharide fractions （ZJP-2, ZJP-4） were obtained. The five fractions all had scavenging capacity for DPPH. Among them, ZJP-5 was the strong scavenger for hydroxyl and superoxide anion radicals, ZJP-2 for superoxide ani- on radical, and ZJP4 for hydroxyl radical. CONCLUSION The macroporous adsorption resin D900 and DEAE- cellulose-52 both have good purifying effect on polysaccharides, and the antioxidant activities of isolated five poly- saccharides have different characteristics.