文献类型：期刊文献

中文题名：白蜡虫脂酰辅酶A还原酶多克隆抗体的制备

英文题名：Preparation of Polyclonal Antibody against Ericerus pela FAR1 Peptide

作者：胡艳红[1,2] 杨璞[2] 陈晓鸣[2] 徐冬丽[2]

第一作者：胡艳红

机构：[1]南京林业大学;[2]中国林业科学研究院资源昆虫研究所

年份：2014

卷号：27

期号：1

起止页码：82-85

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：林业公益性行业科研专项经费项目(201204602)

语种：中文

中文关键词：白蜡虫;脂酰辅酶A还原酶(FAR1);多克隆抗体

外文关键词：Ericerus pela ;fatty acyl-CoAreductase（ FAR1 ）; polyclonal antibody

分类号：S899.1

摘要：白蜡虫脂酰辅酶A还原酶(FAR1)参与了体表蜡泌物的形成过程,为了进一步研究FAR1的功能,通过预测分析基因far1编码的蛋白结构,选取亲水性强、特异性好的一段合成多肽作为抗原肽,免疫新西兰大白兔制备多克隆抗体,并以BCA蛋白浓度试剂盒测定抗体浓度,通过SDS-PAGE考马斯亮蓝染色观察纯化抗体的纯度,以ELISA检测抗体效价。结果表明,纯化所得的多克隆抗体纯度高,抗体浓度0.7 mg·mL-1,抗体效价为1:512 000,为后续FAR基因功能的研究奠定基础。
Fatty acyl-CoA reductase（ FAR1 ）plays a key role in the white wax secretion of Ericerus pela. In present study, the artificially synthesized polypeptide of E. pela FAR1 was used as antigen for the generation of polyclonal antibody. The obtained antibodies were purified by using activated sepharose and analyzed by SDS-PAGE. The con- centration and titer of the polyclonal antibody were determined by BCA KIT and ELISA respectively. The results in- dicated that the titer （ 1：512000） and the concentration （0.7 mg · mL^-1 ） of anti-FAR1 polyclonal antibody could meet the requirements for later experiments, and the polyclonal antibody could be used to further study the location of FAR1 protein in the later research.