文献类型：期刊文献

中文题名：红豆杉低温半致死温度和低温胁迫下紫杉烷含量

英文题名：Semi-lethal Low Temperature and Taxane Content of Taxus Under Low Temperature Stress

作者：蒋路园[1,2] 丰美静[2] 杜雨晴[2] 邸葆[2] 陈段芬[2] 邱德有[1] 杨艳芳[1]

第一作者：蒋路园

机构：[1]中国林业科学研究院林业研究所,北京100091;[2]河北农业大学园艺学院,保定071001

年份：2023

卷号：39

期号：3

起止页码：232-242

中文期刊名：生物技术通报

外文期刊名：Biotechnology Bulletin

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金项目(32171809);国家林业和草原局科技发展中心生物安全与遗传资源管理项目(KJZXSA202037)。

语种：中文

中文关键词：红豆杉;低温胁迫;电阻抗;低温半致死温度;紫杉烷

外文关键词：Taxus;low temperature stress;electrical impedance;semi-lethal low temperature;taxane

分类号：S791.49

摘要：通过测定低温胁迫下红豆杉电阻抗参数和紫杉烷含量变化,确定适合红豆杉低温半致死温度测定的电阻抗参数,探讨低温对红豆杉紫杉烷生物合成的影响,为扩大红豆杉引种范围及利用低温条件提高红豆杉中紫杉醇含量奠定了理论基础。以云南红豆杉、南方红豆杉(山西长治)、中国红豆杉、曼地亚红豆杉和东北红豆杉为材料,分别采用电导率法和电阻抗法测定上述树种低温半致死温度,并利用LC-MS法测定低温胁迫下红豆杉叶片中紫杉烷含量和脱落酸(abscisic acid,ABA)含量。结果表明,5种红豆杉中,中国红豆杉、南方红豆杉(山西长治)、东北红豆杉和曼地亚红豆杉均能耐受-14.668--9.106℃低温;云南红豆杉能耐受-8.802--3.521℃低温,高于其他4种红豆杉。电阻抗τ_(m)参数与电导法测定的5种红豆杉一年生枝低温半致死温度显著相关,相关系数为0.912。低温胁迫下,红豆杉一年生叶片中紫杉醇含量高于当年生叶片。随着处理温度的下降,云南红豆杉和东北红豆杉一年生叶片中紫杉醇含量显著增加,中国红豆杉当年生叶片中巴卡亭Ⅲ含量和10-去乙酰基紫杉醇含量显著增加。此外,红豆杉叶片中ABA含量随温度的下降而增加,当年生叶片中ABA含量高于一年生叶片。与云南红豆杉相比,东北红豆杉、曼地亚红豆杉、南方红豆杉(山西长治)和中国红豆杉能耐受更低的温度。低温有利于云南红豆杉和东北红豆杉一年生叶片中紫杉醇的合成及中国红豆杉当年叶片中巴卡亭Ⅲ和10-去乙酰基紫杉醇的合成。
To determine the electrical impedance parameters suitable for the determination of semi-lethal low temperature of Taxus and explore the effects of low temperature on the taxane biosynthesis in Taxus,the electrical impedance parameters and taxane content changes of Taxus under low temperature stress were examined.It will lay a theoretical foundation for expanding the introduction range of Taxus and using low temperature conditions to increase the contents of taxane content in Taxus.Using T.yunnanensis,T.chinensis var.mairei(Changzhi,Shanxi),T.chinensis,T.cuspidata and T.× media as materials,the semi-lethal low temperature of these trees were determined by conductivity method and electrical impedance method respectively.Moreover,the taxane contents and abscisic acid contents in the leaves of Taxus under low-temperature stress were determined by LC-MS method.The results showed that T.chinensis,T.chinensis var.mairei(Changzhi,Shanxi),T.cuspidata and T.× media all tolerated low temperature of -14.668--9.106℃.The T.yunnanensis tolerated -8.802--3.521℃,which was higher than the ons of other four Taxus species.The semi-lethal low temperature of one-year-old branches of five Taxus measured by electrical impedance τm parameter was significantly correlated with that measured by conductivity method,and the correlation coefficient was 0.912.The taxol content in the leaves of one-year-old Taxus was higher than that in the current-year under low temperature stress.The taxol content in the leaves of one-year-old T.yunnanensis and T.cuspidata significantly increased with the decrease of treatment temperature,while the baccatin Ⅲ content and 10-deacetyltaxol content in the leaves of one-year-old T.chinensis significantly increased.Moreover,ABA content in the leaves of Taxus increased with the decrease of temperature,and ABA content in the current-year leaves was higher than that in the one-yearold leaves.Compared with T.yunnanensis,T.chinensis,T.chinensis var.mairei(Changzhi,Shanxi),T.cuspidata and T.× media tolerated lower temperature.Low temperature is beneficial to the synthesis of taxol in the leaves of one-year-old T.yunnanensis and T.cuspidata,and the synthesis of baccatin Ⅲ and 10-deacetyltaxol in the leaves of current-year T.chinensis.