文献类型：期刊文献

英文题名：Transcriptome-derived microsatellite markers for population diversity analysis in Archidendron clypearia (Jack) IC Nielsen

作者：Li, Dandan[1,2] Li, Mei[2] Li, Fagen[2] Weng, Qijie[2] Zhou, Changpin[2] Huang, Shineng[2] Gan, Siming[1,2]

第一作者：Li, Dandan

通信作者：Gan, SM[1];Gan, SM[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Xiangshan Rd, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Key Lab, Natl Forestry & Grassland Adm Trop Forestry Res, 682 Guangshan Yi Rd, Guangzhou 510520, Guangdong, Peoples R China

年份：2021

卷号：48

期号：12

起止页码：8255-8260

外文期刊名：MOLECULAR BIOLOGY REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000707984100001)】；

基金：This work was financially supported by the Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2018SY019) and the Public Welfare Research and Capacity Building Funds for Social Development from the Department of Science and Technology of Guangdong Province, China (2017A020213002).

语种：英文

外文关键词：Archidendron clypearia (Jack) I; C; Nielsen; Medicinal tree; Transcriptome; Genic microsatellite marker; Genetic diversity

摘要：Background The medicinal woody leguminous genus Archidendron F. Mueller serves as important herbal resources for curing upper respiratory tract infection, acute pharyngitis, tonsillitis, and gastroenteritis. However, genomic resources including transcriptomic sequences and molecular markers remain scarce in the genus. Methods and results Transcriptome sequencing, genic microsatellite marker development, and population diversity analysis were conducted in Archidendron clypearia (Jack) I.C. Nielsen. Flower and flower bud transcriptomes were de novo assembled into 173,172 transcripts, with an average transcript length of 1597.3 bp and an N50 length of 2427 bp. A total of 34,701 microsatellite loci were identified from 26,716 (15.4 %) transcripts. Primer pairs were designed for 718 microsatellite loci, of which 456 (63.5 %) were polymorphic. Of the 456 polymorphic markers, 391 (85.7 %) and 402 (88.1 %) were transferable to A. lucidum (Benth.) I.C. Nielsen and A. multifoliolatum (H.Q. Wen) T.L. Wu, respectively. Using a subset of 15 microsatellite markers, relatively high genetic diversity was detected over two A. clypearia populations, with overall mean expected heterozygosity (H-e) being 0.707 and demonstrating the necessity of conservation. Relatively low differentiation between the two populations was revealed despite the distant separation (about 700 km), with overall inbreeding coefficient of sub-population to the total population (F-st) being 8.7 %. Conclusions This study represents the first attempt to conduct transcriptome sequencing, SSR marker development, and population genetics analysis in the medicinally important genus Archidendron. Our results will offer valuable resources and information for further genetic studies and practical applications in Archidendron and the related taxa.