文献类型：期刊文献

英文题名：Transcriptome comparison analyses in UV-B induced AsA accumulation of Lactuca sativa L

作者：Zhou, Hua[1] Yu, Lei[1,2] Liu, Shujuan[1] Zhu, Anfan[3] Yang, Yanfang[4] Chen, Caihui[1] Yang, Aihong[1] Liu, Lipan[1] Yu, Faxin[1]

第一作者：Zhou, Hua

通信作者：Yu, FX[1]

机构：[1]Jiangxi Acad Sci, Inst Biol Resources, Key Lab Hort Plant Genet & Improvement Jiangxi Pro, Nanchang, Peoples R China;[2]Jiangxi Agr Univ, Coll Forestry, Nanchang 330045, Peoples R China;[3]Jiangxi Agr Technol Extens Ctr, Nanchang 330046, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China

年份：2023

卷号：24

期号：1

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000926228400001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 31960623). There was no additional external funding received for this study. The funder played no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：AsA; UV-B; Dose; Lettuce; Transcriptome

摘要：BackgroundLettuce (Lactuca sativa L.) cultivated in facilities display low vitamin C (L-ascorbic acid (AsA)) contents which require augmentation. Although UV-B irradiation increases the accumulation of AsA in crops, processes underlying the biosynthesis as well as metabolism of AsA induced by UV-B in lettuce remain unclear.ResultsUV-B treatment increased the AsA content in lettuce, compared with that in the untreated control. UV-B treatment significantly increased AsA accumulation in a dose-dependent manner up until a certain dose.. Based on optimization experiments, three UV-B dose treatments, no UV-B (C), medium dose 7.2 KJ center dot m(- 2)center dot d(- 1) (U1), and high dose 12.96 KJ center dot m(- 2)center dot d(- 1) (U2), were selected for transcriptome sequencing (RNA-Seq) in this study. The results showed that C and U1 clustered in one category while U2 clustered in another, suggesting that the effect exerted on AsA by UV-B was dose dependent. MIOX gene in the myo-inositol pathway and APX gene in the recycling pathway in U2 were significantly different from the other two treatments, which was consistent with AsA changes seen in the three treatments, indicating that AsA accumulation caused by UV-B may be associated with these two genes in lettuce. UVR8 and HY5 were not significantly different expressed under UV-B irradiation, however, the genes involved in plant growth hormones and defence hormones significantly decreased and increased in U2, respectively, suggesting that high UV-B dose may regulate photomorphogenesis and response to stress via hormone regulatory pathways, although such regulation was independent of the UVR8 pathway.ConclusionsOur results demonstrated that studying the application of UV-B irradiation may enhance our understanding of the response of plant growth and AsA metabolism-related genes to UV-B stress, with particular reference to lettuce.